Gene Expression Data
Assay Details
Assay
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Reference:
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J:49322
Zuniga A, et al., Gli3 (Xt) and formin (ld) participate in the positioning of the polarising region and control of posterior limb-bud identity. Development. 1999 Jan;126(1):13-21
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1314731
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Gene symbol:
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Fmn1
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Gene name:
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formin 1
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Probe:
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Formin probe1
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Probe preparation:
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labelled with digoxigenin RNA
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Assay notes:
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All results were confirmed by also analyzing ld<ln2>/Xt<H> intercrosses.
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Results
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Specimen
1a:
embryonic day 10.5
(more )
Note: 35 somites.
Specimen
1a:
(close )
Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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35 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud mesenchyme |
Strong |
Graded |
1a
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Expression is highest in posterior-distal mesenchymal cells and decreases towards the anterior in the distal mesenchyme.
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Specimen
1b:
(close )
Genetic Background:
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involves: C57BL/6 * SJL
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
|
embryonic day 10.5
|
Sex:
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Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
35 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud mesenchyme |
Strong |
Graded |
1b
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Expression is highest in posterior-distal mesenchymal cells and decreases towards the anterior in the distal mesenchyme.
|
Specimen
7a E9.5:
embryonic day 9.5
(more )
Note: 25 somites.
Specimen
7a E9.5:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
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25 somites.
|
Specimen
7a E9.75:
embryonic day 9.75
(more )
Note: 28 somites.
Specimen
7a E9.75:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.75
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
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28 somites.
|
Specimen
7b:
embryonic day 10.5
(more )
Note: 34 somites.
Specimen
7b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Note:
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34 somites.
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