Antibody:	anti-Dnmt antibody
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Single cells	1D , 1B	Spermatogonia, preleptotene, and leptotene spermatocytes all express high levels of nuclear Dnmt1, while pachytene spermatocytes are not detectably stained.
TS28: spermatogonium	Present	Single cells	1D , 1B	Spermatogonia, preleptotene, and leptotene spermatocytes all express high levels of nuclear Dnmt1, while pachytene spermatocytes are not detectably stained.

Structure	Level	Pattern	Image	Note
TS27: oocyte	Absent		2A	Non-growing oocytes show no sign of expression.
TS27: ovary	Present	Single cells	2A	Neighboring supporting cells show significant staining.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Single cells	2B , 2C	Growing occytes stain intensely in both nucleus and cytoplasm.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	2E , 2D	Late-stage dictyate occytes in Graafian follicles show very intense and uniform cytoplasmic staining. Optical sections show that Dnmt1 is associated with the oocyte cortex in an ovulated oocyte arrested in metaphase of meiosis II.