Probe:	Dlx-specific probe
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS16: future brain	Present	Regionally restricted	3b	The earliest Dlx transcripts were seen in the region of the optic chiasma.
TS16: diencephalon lateral wall	Present		3b

Structure	Level	Pattern	Image	Note
TS16: future brain	Present	Regionally restricted	3c	The earliest Dlx transcripts were seen in the region of the optic chiasma.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Strong	Regionally restricted	3e	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.
TS20: forebrain	Strong	Regionally restricted	3e	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Strong	Regionally restricted	3f	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.
TS20: forebrain	Strong	Regionally restricted	3f	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.
TS20: diencephalon	Present	Regionally restricted	3f	Dlx is expressed immediately below the zona limitans intrathalamica whose cells do not contain Dlx transcripts. The dorsal limit of the Dlx expression region is therefore orthogonal to the third ventricle and extends dorsally in more lateral areas.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Strong	Regionally restricted	3g	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.
TS20: forebrain	Strong	Regionally restricted	3g	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Strong	Regionally restricted	3h	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.
TS20: forebrain	Strong	Regionally restricted	3h	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Strong	Regionally restricted	3j	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.
TS20: forebrain	Strong	Regionally restricted	3j	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Strong	Regionally restricted	3k	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.
TS20: forebrain	Strong	Regionally restricted	3k	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.
TS20: diencephalon	Present	Regionally restricted	3k	Dlx is expresseed immediately below the zona limitans intrathalamica whose cells do not contain Dlx transcripts. The dorsal limit of the Dlx expression region is therefore orthogonal to the third ventricle and extends dorsally in more lateral areas.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Strong	Regionally restricted	3l	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.
TS20: forebrain	Strong	Regionally restricted	3l	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Strong	Regionally restricted	3n	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.
TS20: forebrain	Strong	Regionally restricted	3n	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.
TS20: diencephalon	Present	Regionally restricted	3n	Dlx is expresseed immediately below the zona limitans intrathalamica whose cells do not contain Dlx transcripts. The dorsal limit of the Dlx expression region is therefore orthogonal to the third ventricle and extends dorsally in more lateral areas.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Strong	Regionally restricted	3o	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.
TS20: forebrain	Strong	Regionally restricted	3o	Dlx is strongly expressed in the striatum germinal zone, with the region in which the transcripts are present extending widely along the anteroposterior and mediolateral axes. Dlx is expressed in very frontal and lateral areas and very posteriorly.
TS20: diencephalon	Present	Regionally restricted	3o	Dlx is expresseed immediately below the zona limitans intrathalamica whose cells do not contain Dlx transcripts. The dorsal limit of the Dlx expression region is therefore orthogonal to the third ventricle and extends dorsally in more lateral areas.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	3p	Dlx is expressed in the entire anterior ventral thalamus in a segment-like pattern.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	3q	Dlx is expresseed immediately below the zona limitans intrathalamica whose cells do not contain Dlx transcripts. The dorsal limit of the Dlx expression region is therefore orthogonal to the third ventricle and extends dorsally in more lateral areas.
TS20: thalamus	Present	Regionally restricted	3q	Dlx is expressed in the entire anterior ventral thalamus in a segment-like pattern.
TS20: hypothalamus	Present	Regionally restricted	3q	In the ventral hypothalamus Dlx transcripts are present in central and lateral areas only in the anterior part.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	3s	Dlx is not expressed either very anteriorly or very posteriorly in the ventral thalamus, but rather in a central area, vertical to the optic chiasma.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	4a	Dlx expression in both the ventral thalamus and the hypothalamus is observed in paraventricular and more lateral areas, but is not detected in the internal germinal layer. Only the external germinal layer and the mantle layer show hybridization.
TS20: thalamus	Present	Regionally restricted	4a	Dlx expression in both the ventral thalamus and the hypothalamus is observed in paraventricular and more lateral areas, but is not detected in the internal germinal layer. Only the external germinal layer and the mantle layer show hybridization.
TS20: diencephalon	Present	Regionally restricted	4a	Dlx is expresseed immediately below the zona limitans intrathalamica whose cells do not contain Dlx transcripts. The dorsal limit of the Dlx expression region is therefore orthogonal to the third ventricle and extends dorsally in more lateral areas

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	4b	Dlx expression in both the ventral thalamus and the hypothalamus is observed in paraventricular and more lateral areas, but is not detected in the internal germinal layer. Only the external germinal layer and the mantle layer show hybridization.
TS20: thalamus	Present	Regionally restricted	4b	Dlx expression in both the ventral thalamus and the hypothalamus is observed in paraventricular and more lateral areas, but is not detected in the internal germinal layer. Only the external germinal layer and the mantle layer show hybridization.
TS20: diencephalon	Present	Regionally restricted	4b	Dlx is expresseed immediately below the zona limitans intrathalamica whose cells do not contain Dlx transcripts. The dorsal limit of the Dlx expression region is therefore orthogonal to the third ventricle and extends dorsally in more lateral areas

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	4c	Dlx expression in both the ventral thalamus and the hypothalamus is observed in paraventricular and more lateral areas, but is not detected in the internal germinal layer. Only the external germinal layer and the mantle layer show hybridization.
TS20: thalamus	Present	Regionally restricted	4c	Dlx expression in both the ventral thalamus and the hypothalamus is observed in paraventricular and more lateral areas, but is not detected in the internal germinal layer. Only the external germinal layer and the mantle layer show hybridization.
TS20: diencephalon	Present	Regionally restricted	4c	Dlx is expresseed immediately below the zona limitans intrathalamica whose cells do not contain Dlx transcripts. The dorsal limit of the Dlx expression region is therefore orthogonal to the third ventricle and extends dorsally in more lateral areas

Structure	Level	Pattern	Image	Note
TS20: neurohypophysis	Absent			The hypothalamus has no transcripts posterior to the optic chiasma, around Rathke's pouch and the region of the future neurohypophysis.
TS20: optic chiasma	Absent			The hypothalamus has no transcripts posterior to the optic chiasma, around Rathke's pouch and the region of the future neurohypophysis.
TS20: remnant of Rathke's pouch	Absent			The hypothalamus has no transcripts posterior to the optic chiasma, around Rathke's pouch and the region of the future neurohypophysis.

Structure	Level	Pattern	Image	Note
TS13: future brain	Absent