Probe:	cf5
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present		3A	Expression is present at the most rostral level of the medial neural plate.

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present		3B	Expression is restricted to the anterior border of the neural plate.

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present	Regionally restricted	3C	Expression is present in the anterior part of the telencephalic neural plate and anterior neural ridge.
TS12: embryo ectoderm	Present	Regionally restricted	3C	Expression is present in the olfactory placode ectoderm. Expression does not extend to the hypophysial placode.
TS12: head mesenchyme	Absent		3C

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	4A	Expression is restricted to the ventral forebrain.
TS15: fronto-nasal process ectoderm	Present	Regionally restricted	4A	Expression is present in a medial band of ectoderm between the developing olfactory placodes, in contact with the ventral forebrain.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	4B	Expression is restricted to the ventral forebrain.
TS15: fronto-nasal process ectoderm	Present	Regionally restricted	4B	Expression is present in a medial band of ectoderm between the developing olfactory placodes, in contact with the ventral forebrain.
TS15: optic stalk	Present	Regionally restricted	4B	Expression is restricted to the rostral & ventral part of the optic stalk.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	4C	Expression is detected in the ventral forebrain.
TS17: optic stalk	Present		4C

Structure	Level	Pattern	Image	Note
TS16: olfactory placode	Present	Regionally restricted	4D	Expression is present in the rostral and medial olfactory placode.
TS16: optic stalk	Present	Regionally restricted	4D	Expression is present in the caudal and rostral part of the optic stalk.
TS16: eye	Present	Regionally restricted	4D	Expression is present in the optic disk. Caudally, expression is limited at the border with the forming infundibulum, corresponding to the developing tuberal hypothalamic region.
TS16: optic cup	Absent		4D

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Present	Regionally restricted	4E	Expression was present in the presumptive anterior oral ectoderm between the olfactory placodes.
TS20: olfactory epithelium	Present		4E	Expression is present in the olfactory placodes.

Structure	Level	Pattern	Image	Note
TS17: oral epithelium	Present	Regionally restricted	4F	Expression was present in the presumptive anterior oral ectoderm between the olfactory placodes.
TS17: olfactory placode	Present		4F
TS17: forebrain	Present	Regionally restricted	4F	Expression is present in the ventral forebrain.