Gene Expression Data
Assay Details
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Assay
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Reference:
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J:50307
Takahashi M, et al., The role of Alx-4 in the establishment of anteroposterior polarity during vertebrate limb development [published erratum appears in Development 1998 Dec;125(23):preceding 4595]. Development. 1998 Nov;125(22):4417-25
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1303147
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Gene symbol:
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Gli3
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Gene name:
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GLI-Kruppel family member GLI3
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Probe:
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Gli3 cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
10 dpc:
embryonic day 10.0
(more  )
Specimen
10 dpc:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud |
Strong |
Regionally restricted |
7A
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Gli3 was highly expressed in the anterior region of the hindlimbs; the posterior region was devoid of detectable Gli3 expression.
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Specimen
12 dpc wt:
embryonic day 12.0
(more )
Specimen
12 dpc wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Present |
Graded |
7B wt
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Gli3 shows a high level of expression in the anterior region, which gradually fades towards the posterior region.
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Specimen
12 dpc lst:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Alx4lst-J/Alx4lst-J
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Present |
Regionally restricted |
7B 1st
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Mutant limb buds lacked detectable Gli3 expression in the most anterior region. The remaining limb region displays a normal expression pattern.
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Specimen
10 dpc wt:
embryonic day 10.0
(more )
Specimen
10 dpc wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud |
Strong |
Regionally restricted |
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Gli3 was highly expressed in the anterior region of the hindlimbs; the posterior region was devoid of detectable Gli3 expression.
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Analysis Tools