Gene Expression Data
Assay Details
Assay
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Reference:
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J:43752
Xiang M, et al., Requirement for Brn-3c in maturation and survival, but not in fate determination of inner ear hair cells. Development. 1998 Oct;125(20):3935-46
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1278004
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Gene symbol:
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Myo7a
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Gene name:
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myosin VIIA
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Antibody:
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anti-myosin VIIa
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Results
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Specimen
Not Shown:
embryonic day 11.5
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: inner ear |
Absent |
|
|
Expression was absent from the hair cells.
|
Specimen
2E:
embryonic day 13.5
(more )
Specimen
2E:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: inner ear |
Weak |
Regionally restricted |
2E
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Expression was detected in hair cells of the otocyst.
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Specimen
2F:
embryonic day 14.5
(more )
Specimen
2F:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: inner ear |
Weak |
Regionally restricted |
2F
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Expression was detected in hair cells of the otocyst.
|
Specimen
Not Shown:
embryonic day 15.5
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cochlea |
Strong |
Regionally restricted |
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Expression was detected in the sensory hair cells.
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TS23: inner ear vestibular component |
Strong |
Regionally restricted |
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Expression was detected in the sensory hair cells.
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Specimen
2G:
embryonic day 16.5
(more )
Specimen
2G:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
2H:
postnatal day 2
(more )
Specimen
2H:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: inner ear |
Strong |
Regionally restricted |
2H
|
Expression was detected in cochlear and vestibular hair cells.
|
Specimen
5A:
embryonic day 15.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: inner ear vestibular component |
Present |
Regionally restricted |
5A
|
Expression was detected in the hair cell layer but was absent from the supporting cell layer.
|
TS23: cochlea |
Present |
Regionally restricted |
5A
|
Expression was detected in the hair cell layer.
|
Specimen
Not Shown:
embryonic day 15.5; Pou4f3tm1Xia/Pou4f3 +
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Mutant Allele(s):
|
Pou4f3tm1Xia/Pou4f3+
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: inner ear vestibular component |
Present |
Regionally restricted |
|
Expression was detected in the hair cell layer but was absent from the supporting cell layer.
|
TS23: cochlea |
Present |
Regionally restricted |
|
Expression was detected in the hair cell layer.
|
Specimen
5B:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
|
Pou4f3tm1Xia/Pou4f3tm1Xia
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: inner ear vestibular component |
Present |
Regionally restricted |
5B
|
Expression was detected in the hair cell layer and in a few of cells in the supporting cell layer.
|
TS23: cochlea |
Present |
Regionally restricted |
5B
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Expression was detected in the hair cell layer.
|
Specimen
5C:
postnatal day 2
(more )
Specimen
5C:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: inner ear |
Present |
Regionally restricted |
5C
|
Expression was detected in the hair cell layer of the cochlear and vestibular systems.
|
Specimen
5D:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
|
Pou4f3tm1Xia/Pou4f3tm1Xia
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: inner ear |
Present |
Regionally restricted |
5D
|
Expression was detected in the hair cell layer of the cochlear and vestibular systems and in a few cells in the supporting cell layer.
|
Specimen
5E:
postnatal day 2
(more )
Specimen
5E:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: inner ear |
Present |
Regionally restricted |
5E
|
Expression was detected in the hair cell layer of the cochlear and vestibular systems.
|
Specimen
5F:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Mutant Allele(s):
|
Pou4f3tm1Xia/Pou4f3tm1Xia
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: inner ear |
Present |
Regionally restricted |
5F
|
Expression was detected in the hair cell layer of the cochlear and vestibular systems and in a few cells in the supporting cell layer.
|
Specimen
5G:
postnatal day 2
(more )
Specimen
5G:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: inner ear |
Present |
Regionally restricted |
5G
|
Expression was detected in the hair cell layer of the cochlear and vestibular systems.
|
Specimen
5H:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Mutant Allele(s):
|
Pou4f3tm1Xia/Pou4f3tm1Xia
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: inner ear |
Present |
Regionally restricted |
5H
|
Expression was detected in the hair cell layer of the cochlear and vestibular systems and in a few cells in the supporting cell layer.
|
Specimen
5I:
postnatal day 2
(more )
Specimen
5I:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: inner ear |
Present |
Regionally restricted |
5I
|
Expression was detected in the hair cell layer of the cochlear and vestibular systems.
|
Specimen
5J:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
|
Pou4f3tm1Xia/Pou4f3tm1Xia
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: inner ear |
Present |
Regionally restricted |
5J
|
Expression was detected in the hair cell layer of the cochlear and vestibular systems and in a few cells in the supporting cell layer.
|
Specimen
5K:
postnatal day 2
(more )
Specimen
5K:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: inner ear |
Present |
Regionally restricted |
5K
|
Expression was detected in the hair cell layer of the cochlear and vestibular systems.
|
Specimen
5L:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Mutant Allele(s):
|
Pou4f3tm1Xia/Pou4f3tm1Xia
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: inner ear |
Present |
Regionally restricted |
5L
|
Expression was detected in the hair cell layer of the cochlear and vestibular systems and in a few cells in the supporting cell layer.
|
Specimen
5M:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Mutant Allele(s):
|
Pou4f3tm1Xia/Pou4f3+
|
Age:
|
postnatal day 8
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: inner ear |
Present |
Regionally restricted |
5M
|
Expression was detected in the hair cell layer of the cochlear and vestibular systems but was absent from the sterociliary bundles on the lumenal surface.
|
Specimen
Not Shown:
postnatal day 8
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Age:
|
postnatal day 8
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: inner ear |
Present |
Regionally restricted |
|
Expression was detected in the hair cell layer of the cochlear and vestibular systems.
|
Specimen
5N:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Mutant Allele(s):
|
Pou4f3tm1Xia/Pou4f3tm1Xia
|
Age:
|
postnatal day 8
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: inner ear |
Present |
Regionally restricted |
5N
|
Expression was detected in the hair cell layer of the cochlear and vestibular systems and in a few cells in the supporting cell layer. Staining was absent from the stereociliary bodies in the cochlear and vestibular labyrinth.
|
|