Gene Expression Data
Assay Details
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Assay
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Reference:
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J:43752
Xiang M, et al., Requirement for Brn-3c in maturation and survival, but not in fate determination of inner ear hair cells. Development. 1998 Oct;125(20):3935-46
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1278003
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Gene symbol:
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Myo6
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Gene name:
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myosin VI
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Antibody:
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anti-myosin VI
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Results
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Specimen
Not Shown:
embryonic day 11.5
(more  )
Specimen
Not Shown:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: inner ear |
Absent |
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Expression was absent from the hair cells.
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Specimen
2A:
embryonic day 13.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: inner ear |
Weak |
Regionally restricted |
2A
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Expression was detected in hair cells of the otocyst.
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Specimen
2B:
embryonic day 14.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: inner ear |
Weak |
Regionally restricted |
2B
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Expression was detected in hair cells of the otocyst.
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Specimen
Not Shown:
embryonic day 15.5
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cochlea |
Strong |
Regionally restricted |
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Expression was detected in the sensory hair cells.
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| TS23: inner ear vestibular component |
Strong |
Regionally restricted |
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Expression was detected in the sensory hair cells.
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Specimen
2C:
embryonic day 16.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2D:
postnatal day 2
(more )
Specimen
2D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: inner ear |
Strong |
Regionally restricted |
2D
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Expression was detected in cochlear and vestibular hair cells.
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Specimen
5O:
postnatal day 2
(more )
Specimen
5O:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: inner ear |
Present |
Regionally restricted |
5O
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Expression was detected in the hair cell layer of the cochlear and vestibular systems.
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Specimen
5P:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f3tm1Xia/Pou4f3tm1Xia
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: inner ear |
Present |
Regionally restricted |
5P
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Expression was detected in a reduced number of hair cells relative to wild type in the sensory epithelium of the cochlear and vestibular systems and a minor fraction of cells within the supporting cell layer of the vestibular system.
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Specimen
Not Shown:
embryonic day 15.5
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cochlea |
Present |
Regionally restricted |
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Expression was detected in the hair cell layer but was absent from the supporting cell layer.
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| TS23: inner ear vestibular component |
Present |
Regionally restricted |
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Expression was detected in the hair cell layer but was absent from the supporting cell layer.
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Specimen
Not Shown:
embryonic day 15.5; Pou4f3tm1Xia/Pou4f3 +
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f3tm1Xia/Pou4f3+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cochlea |
Present |
Regionally restricted |
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Expression was detected in the hair cell layer but was absent from the supporting cell layer.
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| TS23: inner ear vestibular component |
Present |
Regionally restricted |
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Expression was detected in the hair cell layer but was absent from the supporting cell layer.
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Specimen
Not Shown:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f3tm1Xia/Pou4f3tm1Xia
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cochlea |
Present |
Regionally restricted |
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Expression was detected in the hair cells and a minor fraction of cells within the supporting cell layer of the vestibular system.
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| TS23: inner ear vestibular component |
Present |
Regionally restricted |
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Expression was detected in the hair cells and a minor fraction of cells within the supporting cell layer of the vestibular system.
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Analysis Tools