Probe:	M2-3
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS13: head somite	Strong	Graded	4A	Strong expression could be found in the ventral parts of the somites and neural tube, adjacent to the notochord.
TS13: neural tube	Strong	Graded	4A	Strong expression could be found in the ventral parts of the somites and neural tube, adjacent to the notochord.

Structure	Level	Pattern	Image	Note
TS13: head somite	Weak		4B
TS13: neural tube	Weak		4B

Structure	Level	Pattern	Image	Note
TS13: head somite	Strong	Graded	4C	Strong expression could be found in the ventral parts of the somites and neural tube, adjacent to the notochord.
TS13: neural tube	Strong	Graded	4C	Strong expression could be found in the ventral parts of the somites and neural tube, adjacent to the notochord.

Structure	Level	Pattern	Image	Note
TS13: head somite	Weak		4D
TS13: neural tube	Weak		4D

Structure	Level	Pattern	Image	Note
TS13: head somite	Strong	Graded	4E	High levels of expression could be found in the ventral parts of the somites and neural tube, adjacent to the notochord.
TS13: neural tube	Strong	Graded	4E	High levels of expression could be found in the ventral parts of the somites and neural tube, adjacent to the notochord.

Structure	Level	Pattern	Image	Note
TS13: head somite	Weak		4F
TS13: neural tube	Weak		4F

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		4G
TS15: tail neural tube	Present		4G

Structure	Level	Pattern	Image	Note
TS15: embryo	Weak		4H

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Graded	4I	Ventral midline cells have begun to turn off expression.
TS15: tail neural tube	Present	Graded	4I	Ventral midline cells have begun to turn off expression.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	4J	Ventral midline cells of the mutant neural tube still have a fair amount of expression.
TS15: tail neural tube	Present	Regionally restricted	4J	Ventral midline cells of the mutant neural tube still have a fair amount of expression.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Graded	4K	Ventral midline cells have begun to turn off expression.
TS15: tail neural tube	Present	Graded	4K	Ventral midline cells have begun to turn off expression.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	4L	Ventral midline cells of the mutant neural tube still have a fair amount of expression.
TS15: tail neural tube	Present	Regionally restricted	4L	Ventral midline cells of the mutant neural tube still have a fair amount of expression.