Probe:	Fgfr-1 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors did not specify which probe was used in this assay. It is possible MGI:2670369 was used instead.

Structure	Level	Pattern	Image	Note
TS9: embryo mesoderm	Absent		2A
TS9: embryo ectoderm	Absent		2A
TS9: extraembryonic component	Absent		2A

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted	2B	Transcripts were concentrated in the posterior mesoderm, lateral to the primitive streak.
TS10: extraembryonic component	Absent		2B

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted	2C	As gastrulation proceeded, the domain of expression in the mesoderm increased in intensity and expanded anteriorly.
TS10: extraembryonic component	Absent		2C

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted	2D	Dissection of specimens confirmed that expression was expressed in the anterior mesoderm, ventral to the future headfolds.
TS10: embryo ectoderm	Very strong		2D
TS10: extraembryonic component	Absent		2D

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Strong	Regionally restricted	3A	Regionalization of transcripts within the ectoderm was evident; transcripts were NOT detected in the posterior proximolateral ectoderm.
TS11: embryo mesoderm	Present	Regionally restricted	3A	Transcripts were NOT detected in the proximolateral mesoderm.
TS11: extraembryonic component	Absent		3A
TS11: yolk sac mesoderm	Absent		3A
TS11: allantois mesoderm	Absent		3A
TS11: parietal endoderm	Absent		3A
TS11: extraembryonic visceral endoderm	Absent		3A
TS28: decidua	Strong		3A

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Strong	Regionally restricted	3B	Regionalization of transcripts within the ectoderm was evident; transcripts were NOT detected in the posterior proximolateral ectoderm.
TS11: neural ectoderm	Present	Uniform	3B
TS11: future spinal cord neural plate	Strong	Uniform	3B
TS11: embryo mesoderm	Present	Regionally restricted	3B	Transcripts were absent from the anterior mesoderm; transcripts within the mesoderm were localized to the posterior half of the embryo, just lateral to the primitive streak.
TS11: yolk sac mesoderm	Absent		3B
TS11: allantois mesoderm	Absent		3B
TS11: parietal endoderm	Absent		3B
TS11: extraembryonic visceral endoderm	Absent		3B
TS28: decidua	Strong		3B

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	3C	Transcripts were most abundant close to the midline, with the exception of those locations noted elsewhere (primitive streak and node).
TS11: neural ectoderm	Strong	Uniform	3C	Expression was strong in the neurectoderm of the headfolds.
TS11: primitive streak	Strong	Regionally restricted	3C	Transcripts were absent from midline cells of the primitive streak.
TS11: future spinal cord neural plate	Strong		3C
TS11: embryo mesoderm	Present	Regionally restricted	3C	Transcripts within the mesoderm were localized to the posterior half of the embryo, just lateral to the primitive streak. The anterior boundary of transcripts was rostral to the archenteron.
TS11: node	Absent		3C

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	3D	Transcripts were most abundant close to the midline, with the exception of those locations noted elsewhere (primitive streak and node).
TS11: neural ectoderm	Strong	Uniform	3D
TS11: embryo mesoderm	Present	Regionally restricted	3D	Transcripts within the mesoderm were localized to the posterior half of the embryo, just lateral to the primitive streak. Transcripts within the mesoderm showed an anterior boundary just rostral to the archenteron.
TS11: future spinal cord neural plate	Present		3D
TS11: node	Absent		3D
TS11: primitive streak	Present	Regionally restricted	3D	Transcripts were absent from the midline cells of the primitive streak.

Structure	Level	Pattern	Image	Note
TS12: trunk unsegmented mesenchyme	Present	Regionally restricted	3E	A patch of expression marks the site of formation of the next somite prior to its overt segmentation.
TS12: anterior pro-rhombomere neural fold	Strong		3E	Strong expression was seen throughout the neural folds.
TS12: future hindbrain neural fold	Strong		3E	Strong expression was seen throughout the neural folds.
TS12: future midbrain neural fold	Strong		3E	Strong expression was seen throughout the neural folds.
TS12: future prosencephalon neural fold	Strong		3E	Strong expression was seen throughout the neural folds.
TS12: future spinal cord neural fold	Strong		3E	Strong expression was seen throughout the neural folds.
TS12: posterior pro-rhombomere neural fold	Strong		3E	Strong expression was seen throughout the neural folds.

Structure	Level	Pattern	Image	Note
TS13: head somite	Present		3F	Authors note that expression is stronger in the rostral half of a newly formed somite. Within the rostral expression domain, staining appeared to be more intense on the medial side.
TS13: neural tube	Present	Regionally restricted	3F	Levels appeared to gradually decline posteriorly.
TS13: trunk unsegmented mesenchyme	Present		3F

Structure	Level	Pattern	Image	Note
TS12: trunk unsegmented mesenchyme	Present	Regionally restricted	5A	A patch of expression marks the site of formation of the next somite prior to its overt segmentation.
TS12: future prosencephalon	Strong		5A
TS12: heart	Absent		5A

Structure	Level	Pattern	Image	Note
TS13: trunk unsegmented mesenchyme	Present		5B
TS13: trunk somite	Present	Regionally restricted	5B	Expression was observed in the rostral half of the most caudal newly formed epithelial somite.
TS13: future prosencephalon	Strong		5B
TS13: heart	Absent		5B

Structure	Level	Pattern	Image	Note
TS14: head somite	Present	Regionally restricted	5C	More rostral somites were diffusely labeled. The intensity of expression in the rostral half somites decreased prior to the loss of their epithelial arrangement and the subsequent formation of the sclerotome.
TS14: trunk somite	Present	Regionally restricted	5C	More rostral somites were diffusely labeled. The intensity of expression in the rostral half somites decreased prior to the loss of their epithelial arrangement and the subsequent formation of the sclerotome.
TS14: prosencephalon	Strong		5C
TS14: heart	Absent		5C

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Present	Regionally restricted	5D	The dorsal and anterior aspects of the two newest somites were labeled. More rostral somites displayed expression in the forming dermatome.
TS15: heart	Absent		5D
TS15: forelimb bud	Strong		5D
TS15: forelimb bud mesenchyme	Strong		5D
TS15: 1st branchial arch mandibular component mesenchyme	Strong		5D
TS15: 2nd branchial arch mesenchyme	Strong		5D
TS15: branchial arch	Strong		5D
TS15: future brain	Strong		5D
TS15: diencephalon	Very strong		5D