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Gene Expression Data
Assay Details
Assay
Reference: J:19959 Neale SA, et al., Early sialylation on N-CAM in splotch neural tube defect mouse embryos. Teratology. 1994 Aug;50(2):118-24
Assay type: Western blot
MGI Accession ID: MGI:1267094
Gene symbol: Ncam1
Gene name: neural cell adhesion molecule 1
Antibody: anti-N-CAM antibody
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: The proteins in the treated lanes were exposed to N-glycanase enzyme, which removes sialic acid from N-CAM. Antibody detection in the untreated lanes is described as a diffuse smear spanning the region from 140-220 kD corresponding to various N-CAM isoforms.
Results Image: 4
 Sample Information Bands Other Sample Information
Lane AgeStructure 180 kDa 140 kDa (b) Size Not Specified (c) Genetic BackgroundMutant Allele(s)Sex Note
Sp/Sp E9.0 (a) TS14: embryo Present Present Absent Not Specified Pax3Sp/Pax3SpNot Specified N-glycanase treated.
Sp/Sp E9.0 (a) TS14: embryo Not Specified Not Specified Present Not Specified Pax3Sp/Pax3SpNot Specified Untreated.
Sp/+ E9.0 (a) TS14: embryo Present Present Absent Not Specified Pax3Sp/Pax3+Not Specified N-glycanase treated.
Sp/+ E9.0 (a) TS14: embryo Not Specified Not Specified Present Not Specified Pax3Sp/Pax3+Not Specified Untreated.
+/+ E9.0 (a) TS14: embryo Present Present Absent Not Specified Not Specified N-glycanase treated.
+/+ E9.0 (a) TS14: embryo Not Specified Not Specified Present Not Specified Not Specified Untreated.
D14 E14.0 TS22: embryo Present Present Absent C57BL Not Specified N-glycanase treated.
D14 E14.0 TS22: embryo Not Specified Not Specified Present C57BL Not Specified Untreated.
Notes:
(a) 20 somite stage
(b) Authors describe the 140 kD isoform as "a closely spaced doublet which has been reported by others".
(c) Antibody detection is described as "a diffuse smear spanning the region from 140-220 kD corresponding to various N-CAM isoforms."

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory