Gene Expression Data
Assay Details
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Assay
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Reference:
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J:19959
Neale SA, et al., Early sialylation on N-CAM in splotch neural tube defect mouse embryos. Teratology. 1994 Aug;50(2):118-24
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Assay type:
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Western blot
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MGI Accession ID:
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MGI:1267094
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Gene symbol:
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Ncam1
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Gene name:
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neural cell adhesion molecule 1
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Antibody:
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anti-N-CAM antibody
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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The proteins in the treated lanes were exposed to N-glycanase enzyme, which removes sialic acid from N-CAM. Antibody detection in the untreated lanes is described as a diffuse smear spanning the region from 140-220 kD corresponding to various N-CAM isoforms.
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Results
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Image: 4
| E9.0
(a)
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TS14: embryo
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Present
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Present
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Absent
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Not Specified |
Pax3Sp/Pax3Sp | Not Specified |
N-glycanase treated.
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| E9.0
(a)
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TS14: embryo
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Not Specified
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Not Specified
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Present
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Not Specified |
Pax3Sp/Pax3Sp | Not Specified |
Untreated.
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| E9.0
(a)
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TS14: embryo
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Present
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Present
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Absent
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Not Specified |
Pax3Sp/Pax3+ | Not Specified |
N-glycanase treated.
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| E9.0
(a)
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TS14: embryo
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Not Specified
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Not Specified
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Present
|
Not Specified |
Pax3Sp/Pax3+ | Not Specified |
Untreated.
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| E9.0
(a)
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TS14: embryo
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Present
|
Present
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Absent
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Not Specified |
| Not Specified |
N-glycanase treated.
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| E9.0
(a)
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TS14: embryo
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Not Specified
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Not Specified
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Present
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Not Specified |
| Not Specified |
Untreated.
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| E14.0
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TS22: embryo
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Present
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Present
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Absent
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C57BL |
| Not Specified |
N-glycanase treated.
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| E14.0
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TS22: embryo
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Not Specified
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Not Specified
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Present
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C57BL |
| Not Specified |
Untreated.
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Notes:
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Analysis Tools
