Interpreting Probe and Clone Data Details

This help document answers the following questions about the cDNA Clone Source Data Detail report:

• Can I view a more detailed result?
• How do I interpret the detail results of my query?

See also:

• Interpreting a Probe and Clone Data Summary

Can I view a more detailed result?

The summary report provides links to the cDNA Clone Source Data Details. Click the Probe/Clone Acc ID of the desired item to see its detail report.

How do I interpret the detail results of my query?

Each report includes a subset of the following fields, depending on the content of the record. See Primer Detail Results if the sequence type is primer. Note: If the provider did not supply a description for an item, Not Specified appears for this field.

FIELD	DESCRIPTION
Nucleotide Probe/Clone	Name of the probe or segment.
Sequence type	Sequence types are: cDNA, genomic, DNA (construct), mitochondrial, oligo, primer, RNA.
Derivatives	Names of probes/clones derived from the currently displayed probe/clone. Each name is linked to the appropriate child record.
Derived from	Name of probe/clone that the currently displayed probe/clone is derived from. Each name is linked to the appropriate parent record.
Library	Library to which the probe/clone belongs.
Species of origin	Organism from which the probe/clone was derived.
Strain	Strain from which the probe/clone was derived.
Sex	Sex of the specimen from which the probe/clone was derived.
Age	Age of the specimen from which the probe/clone was derived.
Tissue	Tissue of the specimen from which the probe/clone was derived.
Tissue Note	If this is a cell line, the description may detail where the cell line was derived from. If this is a tissue, it may detail the cell type or tissue treatment, if any.
Cell line	Cell line from which the probe/clone was derived
Region covered	Description of the molecular segment inserted into the vector.
Vector type	BAC, cosmid, P1, PAC, phage, phagemid, plasmid, YAC.
Insert site	Restriction site used to insert the segment into the vector.
Insert size	Size of the segment inserted into the vector.
MGI Accession ID	Unique ID assigned to the probe/clone record in MGI. The format is MGI:nnnn (nnnn is any number greater than or equal to 1).
Other MGI Accession IDs	A comma-separated list of any secondary MGI accession IDs associated with the probe/clone.
Other Accession IDs	Unique identifier assigned to the probe/clone by a resource external to the database. For example, in MGI, the RIKEN clone ID is an external accession ID.
Notes	Probe-specific information.

Markers (chromosome):	Symbol for each marker (linked to MGI marker records) to which this probe/clone hybridizes. If the probe is putatively associated with a marker in the MGI database, PUTATIVE appears in parentheses after the marker symbol. The marker's chromosomal assignment appears in parentheses. The marker's genome coordinates appear after the chromosome in the parentheses.
References:	Short-form citations for this probe/clone, each linked to an MGI Reference Detail page. May include notes entered by editors, and, for molecular segments associated with sequence accession numbers, links to GenBank, EMBL, DDBJ, and the MGI Sequence Detail page. If the reference includes sequence, this is also indicated. If polymorphism information is available, it appears in a table with the following column heads: Endonuclease, Marker, Allele, Fragments, Strains.
Additional Information: Gene Expression Data (X results in Y assay)	Expression assays that use the probe.

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Primer Detail Results

If you selected primer as the Sequence Type on the Molecular Probes and Clones Query Form, the first four fields in the detail report describe the primer set. The remainder of the fields contain the information described in detail report above.

FIELD	DESCRIPTION
Primer	Name of the primer set.
Primer 1 sequence	Sequence for the first primer.
Primer 2 sequence	Sequence for the second primer.
Product size	Size of the amplified product.

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