Interpreting Probe and Primer Details
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This help document answers the following questions about the Probe and Primer Detail report:

See also:

How do I interpret the probe details?

Each report includes a subset of the following fields, depending on the content of the record. See Primer Detail Results if the sequence type is primer.

FIELD DESCRIPTION
Nucleotide Probe/Clone
NameName of the probe or segment.
Sequence TypeSequence types are: cDNA, genomic, DNA (construct), mitochondrial, oligo, primer, and Not Specified.
ID Unique MGI ID assigned to the probe/clone record in MGI. The format is MGI:nnnn (nnnn is any number greater than or equal to 1).
Region CoveredDescription of the molecular segment inserted into the vector
Parent CloneName of probe/clone that the currently displayed probe/clone is derived from. Each name is linked to the appropriate parent record.
Child CloneProbes/clones derived from the currently displayed probe/clone. Each name is linked to the appropriate child record.
Vector TypeBAC, cosmid, P1, PAC, phage, phagemid, plasmid, YAC
Insert SiteRestriction site used to insert the segment into the vector
Insert SizeSize of the segment inserted into the vector
Other IDsA list of any secondary MGI accession IDs associated with the probe/clone as well as unique identifiers assigned to the probe/clone by a resource external to the database. Within MGI, ATCC, and IMAGE and RIKEN clone IDs are examples of external accession IDs. These are linked to the appropriate resource when possible. See also: What clones IDs may be associated with a probe?
NoteProbe-specific information
SynonymsAlternative names for the clone used in publications
Source
LibraryLibrary to which the probe/clone belongs
SpeciesOrganism from which the probe/clone was derived
StrainStrain from which the probe/clone was derived
AgeAge of the specimen from which the probe/clone was derived
Sex Sex of the specimen from which the probe/clone was derived
Tissue Tissue of the specimen from which the probe/clone was derived
Tissue Description If this is a cell line, the description may detail where the cell line was derived from. If this is a tissue, it may detail the cell type or tissue treatment, if any.
Cell lineCell line from which the probe/clone was derived
Genes
  • Symbol and name for each gene (linked to MGI gene records) to which this probe/clone hybridizes
  • If the probe is putatively associated with a gene in the MGI database, PUTATIVE appears in parentheses after the gene name.
Expression Links to expression results derived using the probe
Sequences For molecular segments associated with sequence accession numbers, the Accession IDs and links to GenBank, EMBL, DDBJ, and the MGI Sequence Detail page
Polymorphisms For references with polymorphism data, the short-form citation for this probe/clone, linked via an MGI Reference ID (the J:####) to the Reference Detail page. If polymorphism information is available, it appears in a table with the following column heads:
EndonucleaseRestriction enzyme used to cut the DNA.
GeneThe gene or marker the primers amplify
AlleleDesignation for the locus allele in which the polymorphism was found.
FragmentsDescription of the restriction fragments and PCR products
StrainsStrain names for the strains in which the given polymorphism was detected.
References Citations for this probe/clone, each linked via an MGI Reference ID (the J:####) to the Reference Detail page

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How do I interpret the primer details?

If you selected primer as the Sequence Type on a Molecular Probes and Clones Summary page, the first section of the table, Primers, may contain the information the following information as well as information contained in the detail report above.

FIELD DESCRIPTION
NameName of the primer set.
Primer 1 SequenceSequence for the first primer.
Primer 2 SequenceSequence for the second primer.
ID Unique MGI ID assigned to the probe/clone record in MGI. The format is MGI:nnnn (nnnn is any number greater than or equal to 1).
Region CoveredDescription of the molecular segment inserted into the vector
Product SizeSize of the amplified product.
Other IDsA list of any secondary MGI accession IDs associated with the probe/clone as well as unique identifiers assigned to the probe/clone by a resource external to the database. Within MGI, the UniSTS ID is an example of an external accession ID. This are linked to the appropriate resource when possible. See also: What clones IDs may be associated with a probe?
NotePrimer-specific information

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What clones IDs may be associated with a probe?

Potential clone IDs are as follows:

CLONE/PROBE RESOURCELINK
ATCC 
BROAD 
IMAGELinks to the I.M.A.G.E. Consortium Home Page with information on ordering clones
MGCLinks to the Mammalian Gene Collection Home Page with information on ordering clones
NCBI UniSTSLinks to the NCBI STS probe report
NIA 
NIA 7.4K 
NIA 15K 
RIKENLinks to the RIKEN clone sequence information
RIKEN (FANTOM)Links to the RIKEN clone details along with a link for ordering clones
RPCI-23Links to the NCBI Clone Summary with links to distributors
RPCI-24Links to the NCBI Clone Summary with links to distributors
WashU 

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