How do I find existing recombinase-expressing transgenes/knock-ins that have a given promoter (driver)?
Find Recombinase-Carrying Alleles

On most MGI pages, you can access the Recombinase (cre) Activity page from the Search menu as shown in the image at right. From the homepage, you can also access this page by clicking on the "Recombinases (cre)" button.

For this tutorial, open the Recombinase (cre) Activity page in a new window. Scroll down this page for further instructions.

The Access Data section allows you to search for recombinase expression in a specified tissue or under the control of a given gene.

Search Menu

Example 1. Finding a transgene expressing recombinase under the control of Nes.
  1. Type Nes in the Recombinase driven by field. This field has an autocomplete function and you must select a driver if you use this field. Select Nes.

  2. Click Search.
Recombinase driven by
  1. A summary table reporting all cre transgenes driven by the Nes promoter will appear.
    • Note: Transgenes may be experimentally characterized to different degrees, and/or background effects and molecular details may have an impact on recombinase activity within certain tissues.

  2. Recombinase nomenclature takes one of two forms:
    • For randomly inserted transgenes
      Tg(Promoter-Expressed sequence)SerialNumberLabcode where
      • Tg indicates "transgene" (insertion of foreign DNA)
      • Promoter controls expression of the following sequence and takes the syntax of the species of origin (ex. ACTA1 for human, Acta1 for mouse)
      • the expressed protein follows the dash (cre or flp for the most common recombinases in mouse)
      • serial number indicates the line this allele was derived from
      • lab code indicates the research laboratory or institutional project which generated this allele.

    • For targeted transgenes
      TargetedLocustmSerialNumber(expressed sequence)Labcode where
      • targeted locus is an endogenous mouse gene (disrupted), typically lending its promoter to the expressed sequence (cre or flp recombinase) that's been introduced
      • tm indicates "targeted mutation"
      • and expressed protein, serial number and lab code are as above.
  3. Both Recombinase Activity Detected and Recombinase Activity Not Detected are indicated where reported. Sort results by clicking on column headers.
  4. References that have used a given transgene can be found in the References column at the far right of the table, and links to purchasing information can be found by clicking the hyperlinked numbers in the Find Mice (IMSR) column for strains that have been deposited for commercial distribution in repositories indexed by the International Mouse Strain Repository.
  5. Click the Allele Symbol to go to the Transgene Detail Page
Nes Allele results summary
  1. The Transgene Detail page will describe the Mutation Details and summarize the reported Recombinase Activity for the allele. Click the triangles next to row or table features to expand (circles).
  2. Click an individual check mark within the table to open Recombinase Activity detailpages, which display images (where available), patterns of expression, methodology and references associated with each annotation.
  3. Below the Recombinase activity row is a summary table of observed phenotypes for various floxed alleles which have been crossed to the Tg(Nes-cre)1Atp transgene (not shown). See Allelic Composition in the Genotypes table for details.
  4. If you have used this recombinase and have evidence for activity (or no activity) which is not represented in the Recombinase activity table, please submit it to MGI using the form located at the Your Observations Welcome button (arrow).
Nes Allele Recombinase Specificity
  1. The Recombinase Activity Detail page is specific to the selected organ system. In it, you can find links to more detailed data on recombinase gene expression and activity such as level of activity, pattern of expression, assay type used to determine activity and images (where available). The table can be sorted by clicking on column headers.
  2. To view images, click on a corner, hold the mouse down, and drag to resize. The Reset Images button will return them to thumbnails.
Nes Allele Recombinase Activity