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Transgene Detail
Symbol: Tg(CAG-Bgeo,-tTA,-EGFP)2A11Kuw
Name: transgene insertion 2A11, Kay-Uwe Wagner
MGI ID: MGI:3849835
Synonyms: CAG-betageo-tTA, CAG-bGeo-tTA-IRES-GFP, CAG-loxP-betageo-loxP-tTA-IRES-GFP, CAG-tTA-IRES-GFP, pCCALL2-tTA-IRES-GFP
Transgene: Tg(CAG-Bgeo,-tTA,-EGFP)2A11Kuw  Location: unknown  
Alliance: Tg(CAG-Bgeo,-tTA,-EGFP)2A11Kuw page
Germline Transmission:  Earliest citation of germline transmission: J:153278
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Transgene Type:    Transgenic (Conditional ready, Reporter, Transactivator)
Mutation:    Insertion
Mutation detailsThis transgene contains the "CAG promoter" -- consisting of the CMV-IE enhancer followed by the chicken beta-actin (ACTB) upstream promoter, first exon and first intron -- driving expression of a loxP-flanked lacZ/neoR fusion gene (betageo) with three copies of the SV40 polyadenylation signal (3xSV40p[A]). Upon excision of betageo by Cre recombinase, the tetracycline transactivator (tTA) and enhanced green fluorescent protein (EGFP) genes are expressed from the CAG promoter. Presence of the tTA protein promotes expression of other transgenes regulated by the tet operon (tetO); this expression is readily suppressed by doxycycline. (J:153278)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:153278 Zhang Q, et al., Temporally and spatially controlled expression of transgenes in embryonic and adult tissues. Transgenic Res. 2010 Jun;19(3):499-509
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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MGI 6.22
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