Phenotypes Associated with This Genotype

Genotype
MGI:2661905

• Allelic Composition: Ccr7^tm1Rfor/Ccr7^tm1Rfor
• Genetic Background: involves: 129P2/OlaHsd * BALB/c

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal leukocyte migration ( J:57976 )

• adoptive transfer experiments to wild-type recipients revealed that homozygotes display impaired migration of B cells and T cells into lymph nodes and Peyer's pathces, and of T cells into the splenic periarteriolar lymphoid sheath (PALS)

• unlike wild-type B cells, mutant B cells rapidly leave the outer PALS after being transferred into wild-type recipients

impaired B cell migration ( J:57976 )

• adoptive transfer experiments revealed that mutant B cells enter the recipients'lymph nodes and Peyer's patches with a frequency of 20%-50% of that observed after transfer of wild-type cells

• unlike wild-type cells, adoptively transferred mutant B cells are not retained in the outer PALS but quickly migrate to the adjacent follicle, suggesting that temporary retention of B cells in this area and interaction with antigen-specifc T cells is perturbed

enlarged spleen ( J:57976 )

• mutant spleens are usually 2- to 3-fold enlarged relative to wild-type spleens

abnormal class switch recombination ( J:57976 )

• after immunization with the T cell-dependent antigen DNP-KLH, homozygotes exhibit a significant delay in IgG isotype switching relative to similarly-treated wild-type controls

decreased dendritic cell number ( J:57976 )

• unlike in wild-type mice, only few, morphologically altered DCs are detected in the T cell zone of mutant lymph nodes

• following FITC skin painting, significantly less skin-derived DCs are found in mutant LNs relative to wild-type controls

decreased T cell number ( J:57976 )

• homozygotes show a significant reduction in the percentage of CD62L⁺ naive T cells in all secondary lymphoid organs relative to wild-type controls

decreased CD4-positive, alpha-beta T cell number ( J:57976 )

• homozygotes show a significant reduction of CD4⁺ cell numbers in mesenteric and peripheral lymph nodes (50%-70%) and Peyer's patches (25%) relative to wild-type controls

increased T cell number ( J:57976 )

• homozygotes show a significant expansion of CD62L⁺ naive T cells in blood and bone marrow relative to wild-type controls

increased CD4-positive, alpha-beta T cell number ( J:57976 )

• homozygotes show a 6-fold increase in CD4⁺ cell numbers in peripheral blood, spleen, and bone marrow relative to wild-type controls

abnormal spleen red pulp morphology ( J:57976 )

• T cells are mislolocalized and spread throughout the marginal sinuses and the red pulp in large clusters instead of being found in the PALS

abnormal spleen marginal sinus morphology ( J:57976 )

• T cells are mislolocalized and spread throughout the marginal sinuses and the red pulp in large clusters instead of being found in the PALS

abnormal spleen periarteriolar lymphoid sheath morphology ( J:57976 )

• unlike wild-type controls, homozygotes exhibit only very few naive CD62L⁺ T cells in the T cell zone close to the central artery; those T cells found in PALS are of the memory phenotype lacking CD62L expression

• naive T cells reside outside the PALS in mutant mice

abnormal B cell activation ( J:57976 )

• homozygotes display improper activation of B cells, as shown by an increased proportion of IgD^-IgM^low B cells in the germinal center of lymph nodes

• at 10 weeks of age, mutant B cells isolated from blood and all secondary lymphoid organs express higher levels of MHC II molecules than wild-type B cells

• at 3 weeks of age, homozygotes harbor activated B cells (with increased expression levels of MHC II) only in peripheral lymph nodes but not in blood or spleen

abnormal T cell physiology ( J:57976 )

• adoptive transfer experiments revealed that mutant T cells enter the recipients' lymph nodes and Peyer's patches with a frequency of 5% to 25% of that observed after transfer of wild-type cells

• homozygotes display impaired migration of T cells into the splenic periarteriolar lymphoid sheath (PALS)

abnormal Peyer's patch T cell area morphology ( J:57976 )

• mutant Peyer's patches lack T cell-rich zones

• a small rim of T cells is shown to surround the B cell follicle, not observed in wild-type mice

small Peyer's patches ( J:57976 )

• mutant Peyer's patches are smaller than wild-type

abnormal lymph node cortex morphology ( J:57976 )

• homozygotes display an abnormal distribution of B and T cells within the paracortex

abnormal lymph node germinal center morphology ( J:57976 )

• some homozygotes display prominent B cell follicles with significantly enlarged germinal centers in the paracortex

abnormal lymph node T cell domain morphology ( J:57976 )

• T cells are abnormally located toward the marginal sinus

small lymph nodes ( J:57976 )

• mutant lymph nodes are smaller than wild-type

abnormal dendritic cell physiology ( J:57976 )

• following contact sensitization induced by FITC skin painting, homozygotes display impaired migration of activated skin DCs to their draining lumph nodes

decreased susceptibility to type IV hypersensitivity reaction ( J:57976 )

• contact hypersensitivity induced by epicutaneous application of FITC in the ear lobe is completely absent, as assessed by ear swelling at 24 and 48 hrs after reexposure of sensitized homozygotes to the same antigen

• delayed type hypersensitivity induced by s.c. injection of KLH in the ear lobe is completely absent, as assessed by ear swelling at 24 and 48 hrs after reexposure of sensitized homozygotes to the same antigen

abnormal humoral immune response ( J:57976 )

• following application of the T-dependent antigen DNP-KLH, homozygotes fail to produce specific antibodies of any IgG isotype within the first 10 days

increased IgE level ( J:57976 )

increased IgG1 level ( J:57976 )

increased IgG2a level ( J:57976 )

decreased IgG3 level ( J:57976 )

hematopoietic system

abnormal leukocyte migration ( J:57976 )

• adoptive transfer experiments to wild-type recipients revealed that homozygotes display impaired migration of B cells and T cells into lymph nodes and Peyer's pathces, and of T cells into the splenic periarteriolar lymphoid sheath (PALS)

• unlike wild-type B cells, mutant B cells rapidly leave the outer PALS after being transferred into wild-type recipients

impaired B cell migration ( J:57976 )

• adoptive transfer experiments revealed that mutant B cells enter the recipients'lymph nodes and Peyer's patches with a frequency of 20%-50% of that observed after transfer of wild-type cells

• unlike wild-type cells, adoptively transferred mutant B cells are not retained in the outer PALS but quickly migrate to the adjacent follicle, suggesting that temporary retention of B cells in this area and interaction with antigen-specifc T cells is perturbed

enlarged spleen ( J:57976 )

• mutant spleens are usually 2- to 3-fold enlarged relative to wild-type spleens

abnormal class switch recombination ( J:57976 )

• after immunization with the T cell-dependent antigen DNP-KLH, homozygotes exhibit a significant delay in IgG isotype switching relative to similarly-treated wild-type controls

decreased dendritic cell number ( J:57976 )

• unlike in wild-type mice, only few, morphologically altered DCs are detected in the T cell zone of mutant lymph nodes

• following FITC skin painting, significantly less skin-derived DCs are found in mutant LNs relative to wild-type controls

decreased T cell number ( J:57976 )

• homozygotes show a significant reduction in the percentage of CD62L⁺ naive T cells in all secondary lymphoid organs relative to wild-type controls

decreased CD4-positive, alpha-beta T cell number ( J:57976 )

• homozygotes show a significant reduction of CD4⁺ cell numbers in mesenteric and peripheral lymph nodes (50%-70%) and Peyer's patches (25%) relative to wild-type controls

increased T cell number ( J:57976 )

• homozygotes show a significant expansion of CD62L⁺ naive T cells in blood and bone marrow relative to wild-type controls

increased CD4-positive, alpha-beta T cell number ( J:57976 )

• homozygotes show a 6-fold increase in CD4⁺ cell numbers in peripheral blood, spleen, and bone marrow relative to wild-type controls

abnormal spleen red pulp morphology ( J:57976 )

• T cells are mislolocalized and spread throughout the marginal sinuses and the red pulp in large clusters instead of being found in the PALS

abnormal spleen marginal sinus morphology ( J:57976 )

• T cells are mislolocalized and spread throughout the marginal sinuses and the red pulp in large clusters instead of being found in the PALS

abnormal spleen periarteriolar lymphoid sheath morphology ( J:57976 )

• unlike wild-type controls, homozygotes exhibit only very few naive CD62L⁺ T cells in the T cell zone close to the central artery; those T cells found in PALS are of the memory phenotype lacking CD62L expression

• naive T cells reside outside the PALS in mutant mice

abnormal B cell activation ( J:57976 )

• homozygotes display improper activation of B cells, as shown by an increased proportion of IgD^-IgM^low B cells in the germinal center of lymph nodes

• at 10 weeks of age, mutant B cells isolated from blood and all secondary lymphoid organs express higher levels of MHC II molecules than wild-type B cells

• at 3 weeks of age, homozygotes harbor activated B cells (with increased expression levels of MHC II) only in peripheral lymph nodes but not in blood or spleen

increased IgE level ( J:57976 )

increased IgG1 level ( J:57976 )

increased IgG2a level ( J:57976 )

decreased IgG3 level ( J:57976 )

abnormal T cell physiology ( J:57976 )

• adoptive transfer experiments revealed that mutant T cells enter the recipients' lymph nodes and Peyer's patches with a frequency of 5% to 25% of that observed after transfer of wild-type cells

• homozygotes display impaired migration of T cells into the splenic periarteriolar lymphoid sheath (PALS)

cellular

abnormal leukocyte migration ( J:57976 )

• adoptive transfer experiments to wild-type recipients revealed that homozygotes display impaired migration of B cells and T cells into lymph nodes and Peyer's pathces, and of T cells into the splenic periarteriolar lymphoid sheath (PALS)

• unlike wild-type B cells, mutant B cells rapidly leave the outer PALS after being transferred into wild-type recipients

impaired B cell migration ( J:57976 )

• adoptive transfer experiments revealed that mutant B cells enter the recipients'lymph nodes and Peyer's patches with a frequency of 20%-50% of that observed after transfer of wild-type cells

• unlike wild-type cells, adoptively transferred mutant B cells are not retained in the outer PALS but quickly migrate to the adjacent follicle, suggesting that temporary retention of B cells in this area and interaction with antigen-specifc T cells is perturbed

growth/size/body

enlarged spleen ( J:57976 )

• mutant spleens are usually 2- to 3-fold enlarged relative to wild-type spleens