Gene Expression Data
Assay Details
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Assay
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Reference:
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J:60433
Li Song D, et al., Two Pax2/5/8-binding sites in Engrailed2 are required for proper initiation of endogenous mid-hindbrain expression. Mech Dev. 2000 Feb;90(2):155-65
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2388060
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Gene symbol:
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En2
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Gene name:
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engrailed 2
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Probe:
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En2 probe6
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A WT:
embryonic day 8.5
(more  )
Specimen
2A WT:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Moderate |
Regionally restricted |
2A
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Expression is in two triangular patches of cells in the anterior neuroepithelium. The expression level is indicated for comparison the the mutant embryo.
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Specimen
2B En2:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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En2tm3Alj/En2tm3Alj
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Age:
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embryonic day 8.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Weak |
Regionally restricted |
2B
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Expression is in two triangular patches of cells in the anterior neuroepithelium. The pattern of expression in this mutant is the same as in wild-type embryos.
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Specimen
2C WT:
embryonic day 9.5
(more )
Specimen
2C WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2D En2:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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En2tm3Alj/En2tm3Alj
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Weak |
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2D
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The pattern of expression in this mutant is the same as in wild-type embryos.
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| TS15: future midbrain |
Weak |
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2D
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The pattern of expression in this mutant is the same as in wild-type embryos.
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Specimen
E9.5 Not Shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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En2tm3.1Alj/En2tm3.1Alj
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
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Expression in this mutant is the same as in wild-type embryos.
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| TS15: future midbrain |
Present |
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
2E WT:
embryonic day 10.5
(more )
Specimen
2E WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2F En2:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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En2tm3Alj/En2tm3Alj
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Weak |
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2F
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The pattern of expression in this mutant is the same as in wild-type embryos.
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| TS17: midbrain |
Weak |
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2F
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The pattern of expression in this mutant is the same as in wild-type embryos.
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Specimen
E10.5 Not Shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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En2tm3.1Alj/En2tm3.1Alj
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
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Expression in this mutant is the same as in wild-type embryos.
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| TS17: midbrain |
Present |
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
3A WT:
embryonic day 8.5
(more )
Specimen
3A WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Moderate |
Regionally restricted |
3A
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Expression is in two triangular patches of cells in the anterior neuroepithelium.
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Specimen
3B En2:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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En2tm3.1Alj/En2tm3.1Alj
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Age:
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embryonic day 8.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Weak |
Regionally restricted |
3B
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Signal is detected in only the most anterior region of the normal expression domain.
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Specimen
3C WT:
embryonic day 9.0
(more )
Specimen
3C WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3D En2:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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En2tm3.1Alj/En2tm3.1Alj
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Age:
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embryonic day 9.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3E WT:
embryonic day 9.5
(more )
Specimen
3E WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3F En2:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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En2tm3.1Alj/En2tm3.1Alj
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4A En2 RNA:
embryonic day 8.5
(more )
Specimen
4A En2 RNA:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Present |
Regionally restricted |
4A
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Expression is in the anterior neuroepithelium.
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Specimen
4D En2 RNA:
embryonic day 9.0
(more )
Specimen
4D En2 RNA:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4G En2 RNA:
embryonic day 9.5
(more )
Specimen
4G En2 RNA:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4J En2 RNA:
embryonic day 10.5
(more )
Specimen
4J En2 RNA:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
E12.5 Not Shown:
embryonic day 12.5
(more )
Specimen
E12.5 Not Shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
E12.5 Not Shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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En2tm3Alj/En2tm3Alj
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: embryo |
Present |
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
E12.5 Not Shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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En2tm3.1Alj/En2tm3.1Alj
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: embryo |
Present |
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
E15.5 Not Shown:
embryonic day 15.5
(more )
Specimen
E15.5 Not Shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
E15.5 Not Shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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En2tm3Alj/En2tm3Alj
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: embryo |
Present |
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
E17.5 Not Shown:
embryonic day 17.5
(more )
Specimen
E17.5 Not Shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
E17.5 Not Shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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En2tm3Alj/En2tm3Alj
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: embryo |
Present |
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
E17.5 Not Shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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En2tm3.1Alj/En2tm3.1Alj
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: embryo |
Present |
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Expression in this mutant is the same as in wild-type embryos.
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