This document answers the following questions about the Recombinase Alleles - Tissue Activity Summary page:
The Recombinase Alleles Summary presents a table of transgenic and targeted knock-out recombinase alleles contained in the MGI database. It appears when you select either a driver or a system from the boxes on the Recombinase (cre) Activity page and click Go.
The table resulting from a Recombinase Allele Activity query contains a row for either:
The data on the Recombinase Alleles - Tissue Activity Summary are information about expression assays demonstrating the activity of the recombination allele in particular tissues, at or prior to the time when the mouse tissue is assayed. This is referred to as tissue activity or recombinase activity data.
Although all data are captured in MGI's database as expression assays, the assays of type recombinase reporter are more correctly referred to as demonstrating activity of the recombinase allele rather than its expression. This is because tissues where the assay shows recombinase allele activity may be descendents of the tissue (as development and cell differentiation continues) in which the recombinase allele first began producing its protein (began being expressed).
Once the recombinase has acted upon the target gene by removing or rearranging part of its sequence, all descendent tissues will carry the recombined target gene.
Careful analysis of Cre activity in the Cre transgenic mouse lines is important because low levels of excision early in development may produce only a few cells with Cre excision, but all the descendents of those cells will carry the Cre-induced genomic alteration.
Expression assays of type In situ reporter (transgenic) and In situ reporter (knock in) are correctly referred to as expression data because they demonstrate the expression of the recombinase allele at the age when the allele is producing its protein for assays with antibody detection or its mRNA for assays with nucleotide probes. When the specimen's genotype involves a recombinase allele, the results of these expression assays are included in the summary table.Top
The box at the top of the page lets you control the display of the data. See What are the ways of creating different views of the data on this page? for more detail.
|Driver | Allele Symbol/Gene;Allele Name | Recombinase Data | system | Allele Synonym | Allele Type | Inducible | Find Mice | Refs|
|Driver||Gene, genetic element, or construct that promotes recombination.|
| Allele Symbol|
Gene; Allele Name
|Official symbol for the allele.|
Gene name; allele name.
In parentheses beneath the nomenclature is a link to the MGI Detail Report for this allele. Note that the detail page has a Recombinase Activity row listing every system examined where activity has been detected or not detected. Each system links to its activity detail page.
| ||Information about whether recombinase activity data was detected, not detected, or unavailable.|
|Allele Synonym||Any additional designations for the allele.|
|Allele Type||Specific allele type; for example, Transgenic (Cre/Flp), Targeted (knock-in).|
|Inducible||Indicates whether or not treatment (of mice carrying a cre allele) is required to activate recombinase activity. If YES appears, hover over the word to see a pop-up window containing a description of the induction agent (e.g. tamoxifen).|
|Find Mice (IMSR)||Information about the availability of mouse strains and/or cell lines of this allele in the International Mouse Strain Resource (IMSR).|
|Refs||Links to MGI Reference reports for all references annotated to the allele. In parentheses is the total number of reports.|