References
Query Results -- Details
MGI Accession ID: MGI:3055715
J Number: J:93100
Other Accession IDs:
Title: Regulation of cytokine receptors by Golgi N-glycan processing and endocytosis.
Authors: Partridge EA; Le Roy C; Di Guglielmo GM; Pawling J; Cheung P; Granovsky M; Nabi IR; Wrana JL; Dennis JW
Journal: Science
Volume: 306
Issue: 5693
Date: 2004 Oct 1
Year: 2004
Pages: 120-4
Review Status: Peer Reviewed
Abstract:
The Golgi enzyme beta1,6 N-acetylglucosaminyltransferase V (Mgat5) is up-regulated in carcinomas and promotes the substitution of N-glycan with poly N-acetyllactosamine, the preferred ligand for galectin-3 (Gal-3). Here, we report that expression of Mgat5 sensitized mouse cells to multiple cytokines. Gal-3 cross-linked Mgat5-modified N-glycans on epidermal growth factor and transforming growth factor-beta receptors at the cell surface and delayed their removal by constitutive endocytosis. Mgat5 expression in mammary carcinoma was rate limiting for cytokine signaling and consequently for epithelial-mesenchymal transition, cell motility, and tumor metastasis. Mgat5 also promoted cytokine-mediated leukocyte signaling, phagocytosis, and extravasation in vivo. Thus, conditional regulation of N-glycan processing drives synchronous modification of cytokine receptors, which balances their surface retention against loss via endocytosis.
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