References
Query Results -- Details
MGI Accession ID: MGI:3055467
J Number: J:92995
Other Accession IDs:
Title: A transgenic mouse strain expressing four drug-selectable marker genes.
Authors: Tucker KL; Wang Y; Dausman J; Jaenisch R
Journal: Nucleic Acids Res
Volume: 25
Issue: 18
Date: 1997 Sep 15
Year: 1997
Pages: 3745-6
Review Status: Peer Reviewed
Abstract:
Murine embryonic stem (ES) cells are commonly cultured on feeder layers of primary murine embryonic fibroblasts (MEFs). Because gene targeting experiments often involve sequential selection for multiple-drug resistance in single ES cell lines, we have developed a new mouse strain which represents an economical donor for the production of multiple-drug resistant MEFs. MEFs prepared from the DR-4 mouse strain displayed resistance to concentrations of the drugs G418, 6-thioguanine, puromycin and hygromycin well above those used normally for the selection of drug-resistant ES cells.
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