References
Query Results -- Details
MGI Accession ID: MGI:3053258
J Number: J:92520
Other Accession IDs:
Title: p107 regulates neural precursor cells in the mammalian brain.
Authors: Vanderluit JL; Ferguson KL; Nikoletopoulou V; Parker M; Ruzhynsky V; Alexson T; McNamara SM; Park DS; Rudnicki M; Slack RS
Journal: J Cell Biol
Volume: 166
Issue: 6
Date: 2004 Sep 13
Year: 2004
Pages: 853-63
Review Status: Peer Reviewed
Abstract:
Here we show a novel function for Retinoblastoma family member, p107 in controlling stem cell expansion in the mammalian brain. Adult p107-null mice had elevated numbers of proliferating progenitor cells in their lateral ventricles. In vitro neurosphere assays revealed striking increases in the number of neurosphere forming cells from p107(-/-) brains that exhibited enhanced capacity for self-renewal. An expanded stem cell population in p107-deficient mice was shown in vivo by (a) increased numbers of slowly cycling cells in the lateral ventricles; and (b) accelerated rates of neural precursor repopulation after progenitor ablation. Notch1 was up-regulated in p107(-/-) neurospheres in vitro and brains in vivo. Chromatin immunoprecipitation and p107 overexpression suggest that p107 may modulate the Notch1 pathway. These results demonstrate a novel function for p107 that is distinct from Rb, which is to negatively regulate the number of neural stem cells in the developing and adult brain.
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