References
Query Results -- Details
MGI Accession ID: MGI:3052645
J Number: J:92450
Other Accession IDs:
Title: Expression of human apolipoprotein A-II and its effect on high density lipoproteins in transgenic mice.
Authors: Schultz JR; Gong EL; McCall MR; Nichols AV; Clift SM; Rubin EM
Journal: J Biol Chem
Volume: 267
Issue: 30
Date: 1992 Oct 25
Year: 1992
Pages: 21630-6
Review Status: Peer Reviewed
Abstract:
Apolipoproteins A-I and A-II comprise approximately 70 and 20%, respectively, of the total protein content of HDL. Evidence suggests that apoA-I plays a central role in determining the structure and plasma concentration of HDL, while the role of apoA-II is uncertain. To help define the function of apoA-II and determine what effect increasing its plasma concentration has on HDL, transgenic mice expressing human apoA-II and both human apoA-I and human apoA-II were produced. Human apoA-II mRNA is expressed exclusively in the livers of transgenic animals, and the protein exists as a dimer as it does in humans. High level expression of human apoA-II did not increase HDL concentrations or decrease plasma concentrations of murine apoA-I and apoA-II in contrast to what was observed in mice overexpressing human apoA-I. The primary effect of overexpressing human apoA-II was the appearance of small HDL particles composed exclusively of human apoA-II. HDL from mice transgenic for both human apoA-I and human apoA-II displayed a unique size distribution when compared with either apoA-I or apoA-II transgenic mice and contain particles with both these human apolipoproteins. These results in mice, indicating that human apoA-II participates in determining HDL size, parallel results from human studies.
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