References
Query Results -- Details
MGI Accession ID: MGI:2662072
J Number: J:83475
Other Accession IDs:
Title: Notch1 but not Notch2 is essential for generating hematopoietic stem cells from endothelial cells.
Authors: Kumano K; Chiba S; Kunisato A; Sata M; Saito T; Nakagami-Yamaguchi E; Yamaguchi T; Masuda S; Shimizu K; Takahashi T; Ogawa S; Hamada Y; Hirai H
Journal: Immunity
Volume: 18
Issue: 5
Date: 2003 May
Year: 2003
Pages: 699-711
Review Status: Peer Reviewed
Abstract:
Hematopoietic stem cells (HSCs) are thought to arise in the aorta-gonad-mesonephros (AGM) region of embryo proper, although HSC activity can be detected in yolk sac (YS) and paraaortic splanchnopleura (P-Sp) when transplanted in newborn mice. We examined the role of Notch signaling in embryonic hematopoiesis. The activity of colony-forming cells in the YS from Notch1(-/-) embryos was comparable to that of wild-type embryos. However, in vitro and in vivo definitive hematopoietic activities from YS and P-Sp were severely impaired in Notch1(-/-) embryos. The population representing hemogenic endothelial cells, however, did not decrease. In contrast, Notch2(-/-) embryos showed no hematopoietic deficiency. These data indicate that Notch1, but not Notch2, is essential for generating hematopoietic stem cells from endothelial cells.
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