References
Query Results -- Details
MGI Accession ID: MGI:56220
J Number: J:7751
Other Accession IDs:
Title: Dilute-coat-color locus of mice: nucleotide sequence analysis of the d+2J and d+Ha revertant alleles.
Authors: Hutchison KW; Copeland NG; Jenkins NA
Journal: Mol Cell Biol
Volume: 4
Issue: 12
Date: 1984 Dec
Year: 1984
Pages: 2899-904
Review Status: Peer Reviewed
Abstract:
The unstable dilute-coat-color mutation (d) of DBA/2J mice has been shown to be the result of integration of an ecotropic murine leukemia virus within the mouse genome. Molecular cloning and restriction enzyme analysis of the dilute allele and the viral preintegration site (+ allele), as well as two independent dilute revertants (d+2J and d+Ha), suggested that reversion is due to virus excision occurring by homologous recombination involving the viral long terminal repeats. The DNA sequence has now been determined for the cell-virus junctions of the provirus associated with the d mutation, for the viral preintegration site, and for the two revertant sites. These data (i) indicate that the d mutation was caused by a normal virus integration, (ii) confirm that virus excision occurs by precise homologous recombination, as exactly one long terminal repeat is present in each revertant site, and (iii) suggest that the virus induced the d mutation by integration into a noncoding sequence.
Additional Information:
