References
Query Results -- Details
MGI Accession ID: MGI:2176086
J Number: J:75205
Other Accession IDs:
Title: Targeted oncogene activation by site-specific recombination in transgenic mice.
Authors: Lakso M; Sauer B; Mosinger B Jr; Lee EJ; Manning RW; Yu SH; Mulder KL; Westphal H
Journal: Proc Natl Acad Sci U S A
Volume: 89
Issue: 14
Date: 1992 Jul 15
Year: 1992
Pages: 6232-6
Review Status: Peer Reviewed
Abstract:
An efficient and accurate method for controlled in vivo transgene modulation by site-directed recombination is described. Seven transgenic mouse founder lines were produced carrying the murine lens-specific alpha A-crystallin promoter and the simian virus 40 large tumor-antigen gene sequence, separated by a 1.3-kilobase-pair Stop sequence that contains elements preventing expression of the large tumor-antigen gene and Cre recombinase recognition sites. Progeny from two of these lines were mated with transgenic mice expressing the Cre recombinase under control of either the murine alpha A-crystallin promoter or the human cytomegalovirus promoter. All double-transgenic offspring developed lens tumors. Subsequent analysis confirmed that tumor formation resulted from large tumor-antigen activation via site-specific, Cre-mediated deletion of Stop sequences.
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