References
Query Results -- Details
MGI Accession ID: MGI:69361
J Number: J:21372
Other Accession IDs:
Title: Role of leukotrienes revealed by targeted disruption of the 5-lipoxygenase gene.
Authors: Chen XS; Sheller JR; Johnson EN; Funk CD
Journal: Nature
Volume: 372
Issue: 6502
Date: 1994 Nov 10
Year: 1994
Pages: 179-82
Review Status: Peer Reviewed
Abstract:
Leukotrienes constitute a class of potent biological mediators of inflammation and anaphylaxis (for reviews see refs 1 and 2). Their biosynthesis derives from 5-lipoxygenase-catalysed oxygenation of arachidonic acid in granulocytes, macrophages and mast cells. To examine the physiological importance of leukotrienes, we have disrupted the 5-lipoxygenase gene by homologous recombination in embryonic stem cells. 5-Lipoxygenase-deficient (5LX-/-) mice develop normally and are healthy. They show a selective opposition to certain inflammatory insults. Although there is no difference in their reaction to endotoxin shock, the 5LX-/- animals resist the lethal effects of shock induced by platelet-activating factor. Reaction to ear inflammation induced by phorbol ester is normal, whereas inflammation induced by arachidonic acid is markedly reduced. Contrasts were also found in two models of leukocyte chemotaxis in vivo. The phenotype of 5LX-/- mice under injurious insult identifies the role for leukotrienes in the pathophysiology of select inflammatory states.
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