References
Query Results -- Details
MGI Accession ID: MGI:3840767
J Number: J:147475
Other Accession IDs:
Title: Amygdala protein kinase C epsilon regulates corticotropin-releasing factor and anxiety-like behavior.
Authors: Lesscher HM; McMahon T; Lasek AW; Chou WH; Connolly J; Kharazia V; Messing RO
Journal: Genes Brain Behav
Volume: 7
Issue: 3
Date: 2008 Apr
Year: 2008
Pages: 323-33
Review Status: Peer Reviewed
Abstract:
Corticotropin-releasing factor (CRF), its receptors, and signaling pathways that regulate CRF expression and responses are areas of intense investigation for new drugs to treat affective disorders. Here, we report that protein kinase C epsilon (PKCepsilon) null mutant mice, which show reduced anxiety-like behavior, have reduced levels of CRF messenger RNA and peptide in the amygdala. In primary amygdala neurons, a selective PKCepsilon activator, psiepsilonRACK, increased levels of pro-CRF, whereas reducing PKCepsilon levels through RNA interference blocked phorbol ester-stimulated increases in CRF. Local knockdown of amygdala PKCepsilon by RNA interference reduced anxiety-like behavior in wild-type mice. Furthermore, local infusion of CRF into the amygdala of PKCepsilon(-/-) mice increased their anxiety-like behavior. These results are consistent with a novel mechanism of PKCepsilon control over anxiety-like behavior through regulation of CRF in the amygdala.
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