References
Query Results -- Details
MGI Accession ID: MGI:3806207
J Number: J:138731
Other Accession IDs:
Title: Separable requirements for cytoplasmic domain of PSGL-1 in leukocyte rolling and signaling under flow.
Authors: Miner JJ; Xia L; Yago T; Kappelmayer J; Liu Z; Klopocki AG; Shao B; McDaniel JM; Setiadi H; Schmidtke DW; McEver RP
Journal: Blood
Volume: 112
Issue: 5
Date: 2008 Sep 1
Year: 2008
Pages: 2035-45
Review Status: Peer Reviewed
Abstract:
In inflamed venules, leukocytes use P-selectin glycoprotein ligand-1 (PSGL-1) to roll on P-selectin and E-selectin and to activate integrin alphaLbeta2 (lymphocyte function-associated antigen-1, LFA-1) to slow rolling on intercellular adhesion molecule-1 (ICAM-1). Studies in cell lines have suggested that PSGL-1 requires its cytoplasmic domain to localize in membrane domains, to support rolling on P-selectin, and to signal through spleen tyrosine kinase (Syk). We generated 'DeltaCD' mice that express PSGL-1 without the cytoplasmic domain. Unexpectedly, neutrophils from these mice localized PSGL-1 normally in microvilli, uropods, and lipid rafts. DeltaCD neutrophils expressed less PSGL-1 on their surfaces because of inefficient export from the endoplasmic reticulum. Limited digestion of wild-type neutrophils with O-sialoglycoprotein endopeptidase was used to reduce the PSGL-1 density to that on DeltaCD neutrophils. At matched PSGL-1 densities, both DeltaCD and wild-type neutrophils rolled similarly on P-selectin. However, DeltaCD neutrophils rolling on P-selectin did not trigger Syk-dependent activation of LFA-1 to slow rolling on ICAM-1. These data demonstrate that the PSGL-1 cytoplasmic domain is dispensable for leukocyte rolling on P-selectin but is essential to activate beta2 integrins to slow rolling on ICAM-1.
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