References
Query Results -- Details
MGI Accession ID: MGI:3798345
J Number: J:137220
Other Accession IDs:
Title: Protein kinase C epsilon mediation of CRF- and ethanol-induced GABA release in central amygdala.
Authors: Bajo M; Cruz MT; Siggins GR; Messing R; Roberto M
Journal: Proc Natl Acad Sci U S A
Volume: 105
Issue: 24
Date: 2008 Jun 17
Year: 2008
Pages: 8410-5
Review Status: Peer Reviewed
Abstract:
In the central amygdala (CeA), ethanol acts via corticotrophin-releasing factor (CRF) type 1 receptors to enhance GABA release. Amygdala CRF mediates anxiety associated with stress and drug dependence, and it regulates ethanol intake. Because mutant mice that lack PKCepsilon exhibit reduced anxiety-like behavior and alcohol consumption, we investigated whether PKCepsilon lies downstream of CRF(1) receptors in the CeA. Compared with PKCepsilon(+/+) CeA neurons, PKCepsilon(-/-) neurons showed increased GABAergic tone due to enhanced GABA release. CRF and ethanol stimulated GABA release in the PKCepsilon(+/+) CeA, but not in the PKCepsilon(-/-) CeA. A PKCepsilon-specific inhibitor blocked both CRF- and ethanol-induced GABA release in the PKCepsilon(+/+) CeA, confirming findings in the PKCepsilon(-/-) CeA. These results identify a PKCepsilon signaling pathway in the CeA that is activated by CRF(1) receptor stimulation, mediates GABA release at nerve terminals, and regulates anxiety and alcohol consumption.
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