References
Query Results -- Details
MGI Accession ID: MGI:3795792
J Number: J:136249
Other Accession IDs:
Title: Ablation of estrogen receptor alpha (ERalpha) prevents upregulation of POMC by leptin and insulin.
Authors: Hirosawa M; Minata M; Harada KH; Hitomi T; Krust A; Koizumi A
Journal: Biochem Biophys Res Commun
Volume: 371
Issue: 2
Date: 2008 Jun 27
Year: 2008
Pages: 320-3
Review Status: Peer Reviewed
Abstract:
Diabetic Akita male mice are more hyperphagic because of downregulation of proopiomelanocortin (POMC) caused by hypoleptinemia. We investigated the role of estrogen receptor alpha (ERalpha) in the regulation of the hypothalamic POMC in females. ERaKOAkt mice consumed 30% greater food (g/3 weeks) than the Akita diabetic controls. Ovariectomized diabetic (AFO) and nondiabetic (B6FO) mice had significantly lower food intake and elevated serum leptin levels. ERaKOAkt and ERaKO mice also increased serum leptin concentrations, while hypoinsulinemia was observed in ERaKOAkt and hyperinsulinemia in ERaKO mice. RT-PCR showed a significant attenuation of POMC expression in both ERaKOAkt and ERaKO mice, irrespective of the elevated leptin serum levels or hyperinsulinemia, while elevated serum leptin levels in AFO and B6FO mice upregulated POMC gene expression. These results indicate that ERalpha plays an essential role in leptin- and insulin-stimulated upregulation of the POMC gene. This action of ERalpha is likely mediated in a ligand-independent manner.
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