References
Query Results -- Details
MGI Accession ID: MGI:3774709
J Number: J:131841
Other Accession IDs:
Title: Loss of expression of N-acetylglucosaminyltransferase Va results in altered gene expression of glycosyltransferases and galectins.
Authors: Guo HB; Nairn A; Harris K; Randolph M; Alvarez-Manilla G; Moremen K; Pierce M
Journal: FEBS Lett
Volume: 582
Issue: 4
Date: 2008 Feb 20
Year: 2008
Pages: 527-35
Review Status: Peer Reviewed
Abstract:
We isolated mouse embryo fibroblasts (MEFs) from N-acetylglucosaminyltransferase Va (GnT-Va) knockout mice and studied the effects of loss of expression of GnT-Va on asparagine-linked glycans (N-glycan) synthesis and the gene expression of groups of glycosyltransferases and galectins. Loss of GnT-Va expression caused aberrant expression of several N-glycan structures, including N-linked beta(1,6) branching, poly-N-lactosamine, bisecting N-acetylglucosamine (GlcNAc) and sialic acid. Using quantitative reverse transcriptase-PCR (qRT-PCR), altered gene expression of several groups of glycosyltransferases and galectins was observed in GnT-Va null MEFs, supporting the observed changes in N-glycan structures. These results suggest that genetic disruption of GnT-Va ultimately resulted in altered MEFs gene expression and decreased tumor progression associated with loss of GnT-Va observed may result in part from a combination of effects from these altered gene expressions.
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