References
Query Results -- Details
MGI Accession ID: MGI:3771325
J Number: J:130250
Other Accession IDs:
Title: In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state.
Authors: Wernig M; Meissner A; Foreman R; Brambrink T; Ku M; Hochedlinger K; Bernstein BE; Jaenisch R
Journal: Nature
Volume: 448
Issue: 7151
Date: 2007 Jul 19
Year: 2007
Pages: 318-24
Review Status: Peer Reviewed
Abstract:
Nuclear transplantation can reprogramme a somatic genome back into an embryonic epigenetic state, and the reprogrammed nucleus can create a cloned animal or produce pluripotent embryonic stem cells. One potential use of the nuclear cloning approach is the derivation of 'customized' embryonic stem (ES) cells for patient-specific cell treatment, but technical and ethical considerations impede the therapeutic application of this technology. Reprogramming of fibroblasts to a pluripotent state can be induced in vitro through ectopic expression of the four transcription factors Oct4 (also called Oct3/4 or Pou5f1), Sox2, c-Myc and Klf4. Here we show that DNA methylation, gene expression and chromatin state of such induced reprogrammed stem cells are similar to those of ES cells. Notably, the cells-derived from mouse fibroblasts-can form viable chimaeras, can contribute to the germ line and can generate live late-term embryos when injected into tetraploid blastocysts. Our results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells.
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