References
Query Results -- Details
MGI Accession ID: MGI:3766051
J Number: J:128069
Other Accession IDs:
Title: High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells.
Authors: Raymond CS; Soriano P
Journal: PLoS ONE
Volume: 2
Issue: 1
Date: 2007
Year: 2007
Pages: e162
Review Status: Peer Reviewed
Abstract:
DNA site-specific recombinases (SSRs) such as Cre, FLPe, and phiC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and PhiC31 (PhiC31o) SSRs, which result in recombination efficiencies similar to Cre.
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