References
Query Results -- Details
MGI Accession ID: MGI:3764582
J Number: J:127660
Other Accession IDs:
Title: Long-term, noninvasive imaging of regulated gene expression in living mice.
Authors: Hasan MT; Schonig K; Berger S; Graewe W; Bujard H
Journal: Genesis
Volume: 29
Issue: 3
Date: 2001 Mar
Year: 2001
Pages: 116-22
Review Status: Peer Reviewed
Abstract:
We describe here an approach for monitoring regulated gene expression by noninvasive imaging in living mice. We have utilized the tetracycline inducible system to simultaneously coregulate the expression of two genes encoding the firefly luciferase and the Cre recombinase, respectively. Results from our model system demonstrate that luciferase can be used as a noninvasive imaging marker for the regulated expression of a second gene in living mice. The integration of noninvasive imaging and inducible gene expression into current approaches of functional genomics should greatly advance our capabilities of carrying out highly controlled long-term studies of gene function in individual mice.
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