References
Query Results -- Details
MGI Accession ID: MGI:3719544
J Number: J:123796
Other Accession IDs:
Title: Differentially regulated expression and function of CD22 in activated B-1 and B-2 lymphocytes.
Authors: Lajaunias F; Nitschke L; Moll T; Martinez-Soria E; Semac I; Chicheportiche Y; Parkhouse RM; Izui S
Journal: J Immunol
Volume: 168
Issue: 12
Date: 2002 Jun 15
Year: 2002
Pages: 6078-83
Review Status: Peer Reviewed
Abstract:
CD22 is a B cell-restricted transmembrane protein that apparently controls signal transduction thresholds initiated through the B cell Ag receptor (BCR) in response to Ag. However, it is still poorly understood how the expression of CD22 is regulated in B cells after their activation. Here we show that the expression levels of CD22 in conventional B-2 cells are markedly down-regulated after cross-linking of BCR with anti-IgM mAb but are up-regulated after stimulation with LPS, anti-CD40 mAb, or IL-4. In contrast, treatment with anti-IgM mAb barely modulated the expression levels of CD22 in CD5(+) B-1 cells, consistent with a weak Ca(2+) response in anti-IgM-treated CD5(+) B-1 cells. Moreover, in CD22-deficient mice, anti-IgM treatment did not trigger enhanced Ca(2+) influx in CD5(+) B-1 cells, unlike CD22-deficient splenic B-2 cells, suggesting a relatively limited role of CD22 in BCR signaling in B-1 cells. In contrast, CD22 levels were markedly down-regulated on wild-type B-1 cells in response to LPS or unmethylated CpG-containing oligodeoxynucleotides. These data indicate that the expression and function of CD22 are differentially regulated in B-1 and conventional B-2 cells, which are apparently implicated in innate and adaptive immunity, respectively.
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