References
Query Results -- Details
MGI Accession ID: MGI:3712389
J Number: J:121839
Other Accession IDs:
Title: Carbonic anhydrase XIV deficiency produces a functional defect in the retinal light response.
Authors: Ogilvie JM; Ohlemiller KK; Shah GN; Ulmasov B; Becker TA; Waheed A; Hennig AK; Lukasiewicz PD; Sly WS
Journal: Proc Natl Acad Sci U S A
Volume: 104
Issue: 20
Date: 2007 May 15
Year: 2007
Pages: 8514-9
Review Status: Peer Reviewed
Abstract:
Members of the carbonic anhydrase (CA) family play an important role in the regulation of pH, CO(2), ion, and water transport. CA IV and CA XIV are membrane-bound isozymes expressed in the eye. CA IV immunostaining is limited to the choriocapillaris overlying the retina, whereas CA XIV is expressed within the retina in Muller glial cells and retinal pigment epithelium. Here, we have characterized the physiological and morphological phenotype of the CA IV-null, CA XIV-null, and CA IV/CA XIV-double-null mouse retinas. Flash electroretinograms performed at 2, 7, and 10 months of age showed that the rod/cone a-wave, b-wave, and cone b-wave were significantly reduced (26-45%) in the CA XIV-null mice compared with wild-type littermates. Reductions in the dark-adapted response were not progressive between 2 and 10 months, and no differences in retinal morphology were observed between wild-type and CA XIV-null mice. Muller cells and rod bipolar cells had a normal appearance. Retinas of CA IV-null mice showed no functional or morphological differences compared with normal littermates. However, CA IV/CA XIV double mutants showed a greater deficit in light response than the CA XIV-null retina. Our results indicate that CA XIV, which regulates extracellular pH and pCO(2), plays an important part in producing a normal retinal light response. A larger functional deficit in the CA IV/CA XIV double mutants suggests that CA IV can also contribute to pH regulation, at least in the absence of CA XIV.
Additional Information:
