References
Query Results -- Details
MGI Accession ID: MGI:3709527
J Number: J:121210
Other Accession IDs:
Title: Surface tension influences cell shape and phagocytosis in alveolar macrophages.
Authors: Akei H; Whitsett JA; Buroker M; Ninomiya T; Tatsumi H; Weaver TE; Ikegami M
Journal: Am J Physiol Lung Cell Mol Physiol
Volume: 291
Issue: 4
Date: 2006 Oct
Year: 2006
Pages: L572-9
Review Status: Peer Reviewed
Abstract:
The effect of surface tension on alveolar macrophage shape and phagocytosis was assessed in vivo and in vitro. Surface tension was regulated in vivo by conditionally expressing surfactant protein (SP)-B in Sftpb-/- mice. Increased surface tension and respiratory distress were produced by depletion of SP-B and were readily reversed by repletion of SP-B in vivo. Electron microscopy was used to demonstrate that alveolar macrophages were usually located beneath the surfactant film on the alveolar surfaces. Reduction of SP-B increased surface tension and resulted in flattening of alveolar macrophages on epithelial surfaces in vivo. Phagocytosis of intratracheally injected fluorescent microbeads by alveolar macrophages was decreased during SP-B deficiency and was restored by repletion of SP-B in vivo. Incubation of MH-S cells, a mouse macrophage cell line, with inactive surfactant caused cell flattening and decreased phagocytosis in vitro, findings that were reversed by the addition of sheep surfactant or phospholipid containing SP-B. SP-B controls surface tension by forming a surfactant phospholipid film that regulates shape and nonspecific phagocytic activity of alveolar macrophages on the alveolar surface.
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