References
Query Results -- Details
MGI Accession ID: MGI:3707618
J Number: J:120653
Other Accession IDs:
Title: Severe inflammation and reduced bacteria load in murine helicobacter infection caused by lack of phagocyte oxidase activity.
Authors: Blanchard TG; Yu F; Hsieh CL; Redline RW
Journal: J Infect Dis
Volume: 187
Issue: 10
Date: 2003 May 15
Year: 2003
Pages: 1609-15
Review Status: Peer Reviewed
Abstract:
The vaccine-induced immune mechanisms that protect against Helicobacter pylori infection in the mouse model have not been identified. This study investigated the contribution of reactive oxygen and nitrogen intermediates to Helicobacter pathogenesis and immunity. Mice deficient in nicotinamide-adenine dinucleotide phosphate oxidase activity (gp91(phox-/-)), nitric oxide synthase activity (NOS2(-/-)), or both (gp91(phox-/-)/NOS2(-/-)) were infected with Helicobacter organisms and evaluated for inflammation and bacteria load. Infection of all 3 transgenic strains resulted in significantly more inflammation than found in infected C57BL/6 wild-type mice. However, only gp91(phox-/-) and gp91(phox-/-)/NOS2(-/-) mice had significantly reduced numbers of infected gastric glands. Intranasal immunization of NOS2(-/-) or gp91(phox-/-)/NOS2(-/-) mice against H. pylori resulted in protective immunity comparable to that seen in C57BL/6 control mice. Therefore, reactive oxygen species may play a role in limiting the inflammatory response associated with H. pylori infection of the gastric mucosa but may also limit the host's ability to eradicate Helicobacter organisms.
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