References
Query Results -- Details
MGI Accession ID: MGI:3655592
J Number: J:112153
Other Accession IDs:
Title: Inducible-NOS but not neuronal-NOS participate in the acute effect of TNF-alpha on hypothalamic insulin-dependent inhibition of food intake.
Authors: Moraes JC; Amaral ME; Picardi PK; Calegari VC; Romanatto T; Bermudez-Echeverry M; Chiavegatto S; Saad MJ; Velloso LA
Journal: FEBS Lett
Volume: 580
Issue: 19
Date: 2006 Aug 21
Year: 2006
Pages: 4625-31
Review Status: Peer Reviewed
Abstract:
TNF-alpha acts on the hypothalamus modulating food intake and energy expenditure through mechanisms incompletely elucidated. Here, we explore the hypothesis that, to modulate insulin-induced anorexigenic signaling in hypothalamus, TNF-alpha requires the synthesis of NO. TNF-alpha activates signal transduction through JNK and p38 in hypothalamus, peaking at 10(-8) M. This is accompanied by the induction of expression of the inducible and neuronal forms of NOS, in both cases peaking at 10(-12) M. In addition, TNF-alpha stimulates NOS catalytic activity. Pre-treatment with TNF-alpha at a low dose (10(-12) M) inhibits insulin-dependent anorexigenic signaling, and this effect is abolished in iNOS but not in nNOS knockout mice.
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