References
Query Results -- Details
MGI Accession ID: MGI:3652691
J Number: J:111070
Other Accession IDs:
Title: Regulation of large dense-core vesicle volume and neurotransmitter content mediated by adaptor protein 3.
Authors: Grabner CP; Price SD; Lysakowski A; Cahill AL; Fox AP
Journal: Proc Natl Acad Sci U S A
Volume: 103
Issue: 26
Date: 2006 Jun 27
Year: 2006
Pages: 10035-40
Review Status: Peer Reviewed
Abstract:
Adaptor protein 3 (AP-3) is a vesicle-coat protein that forms a heterotetrameric complex. Two types of AP-3 subunits are found in mammalian cells. Ubiquitous AP-3 subunits are expressed in all tissues of the body, including the brain. In addition, there are neuronal AP-3 subunits that are thought to serve neuron-specific functions such as neurotransmitter release. In this study, we show that overexpression of neuronal AP-3 in mouse chromaffin cells results in a striking decrease in the neurotransmitter content of individual vesicles (quantal size), whereas deletion of all AP-3 produces a dramatic increase in quantal size; these changes were correlated with alterations in dense-core vesicle size. AP-3 appears to localize in the trans-Golgi network and possibly immature secretory vesicles, where it may be involved in the formation of neurosecretory vesicles.
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