References
Query Results -- Details
MGI Accession ID: MGI:59580
J Number: J:11138
Other Accession IDs:
Title: Direct molecular identification of the mouse pink-eyed unstable mutation by genome scanning.
Authors: Brilliant MH; Gondo Y; Eicher EM
Journal: Science
Volume: 252
Issue: 5005
Date: 1991 Apr 26
Year: 1991
Pages: 566-9
Review Status: Peer Reviewed
Abstract:
DNA sequences associated with the mouse pink-eyed unstable mutation were identified in the absence of closely linked molecular markers and without prior knowledge of the encoded gene product. This was accomplished by genome scanning, a technique in which high-resolution Southern blots of genomic DNAs were hybridized to a dispersed and moderately repetitive DNA sequence. In this assay, pink-eyed unstable DNA was distinguished from the DNA of wild-type and revertant mice by enhanced hybridization to one of several hundred resolved fragments. The fragment showing enhanced hybridization in pink-eyed unstable DNA was cloned and found to lie within a DNA duplication that is located close to, or within, the pink-eyed dilution locus. The duplication associated with the mouse pink-eyed unstable mutation may mediate the high reversion frequency characteristic of this mutation.
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