References
Query Results -- Details
MGI Accession ID: MGI:3620259
J Number: J:107062
Other Accession IDs:
Title: Regulation of B cell development and B cell signalling by CD22 and its ligands {alpha}2,6-linked sialic acids.
Authors: Ghosh S; Bandulet C; Nitschke L
Journal: Int Immunol
Volume: 18
Issue: 4
Date: 2006 Apr
Year: 2006
Pages: 603-11
Review Status: Peer Reviewed
Abstract:
CD22 is an inhibitory co-receptor of B cell receptor (BCR)-mediated signalling which binds specifically to glycan ligands containing alpha2,6-linked sialic acids. This interaction modulates the CD22 activity by an unknown mechanism. Mice deficient for ST6GalI, the enzyme that generates alpha2,6-linked sialic acids, show an immunodeficient and opposing phenotype to CD22-deficient mice. By generating mice double-deficient for this receptor/ligand pair, we analysed its influence on B cell maturation and signalling. Both ST6GalI-deficient and ST6GalI x CD22-deficient mice showed normal B cell development, but an impaired marginal zone B cell population in the spleen. Both types of mutant mice also showed a reduced population of bone marrow recirculating B cells, a defect previously detected in CD22(-/-) mice. In adoptive transfer experiments, a migration defect of wild-type B cells to the bone marrow of ST6GalI-deficient mice was found. This suggests a direct involvement of CD22 and its ligands 2,6Sia in a homing process of recirculating B cells to the bone marrow. Interestingly, defective B cell Ca(2+) signalling and proliferation of ST6Gal(-/-) mice was rescued in ST6GalI x CD22-deficient mice. This points to a new mechanism of BCR signal regulation by CD22 and its ligand.
Additional Information:
