References
Query Results -- Details
MGI Accession ID: MGI:3586864
J Number: J:100079
Other Accession IDs:
Title: Human coxsackie adenovirus receptor (CAR) expression in transgenic mouse prostate tumors enhances adenoviral delivery of genes.
Authors: Bao Y; Peng W; Verbitsky A; Chen J; Wu L; Rauen KA; Sawicki JA
Journal: Prostate
Volume: 64
Issue: 4
Date: 2005 Sep 1
Year: 2005
Pages: 401-7
Review Status: Peer Reviewed
Abstract:
BACKGROUND: Transgenic mice that recapitulate the progression of human diseases are potentially useful models for testing the effectiveness of new therapeutic strategies. Their use in pre-clinical testing of adenovirally-delivered gene therapies, however, is limited because of restricted cell surface expression of Coxsackie adenovirus receptor (CAR) in mice. METHODS: To develop a more suitable transgenic mouse model for testing adenoviral-based gene therapies for prostate cancer, we generated prostate specific antigen/human CAR (PSA/hCAR) transgenic mice in which a chimeric enhancer/promoter sequence of the human PSA gene drives expression of a functional hCAR coding sequence. RESULTS: Expression of an adenovirally-delivered luciferase reporter gene in prostate tumor cells in bigenic mice (PSA/hCAR + TRAMP) was enhanced compared to the level in tumor cells lacking the PSA/hCAR transgene. CONCLUSIONS: Breeding PSA/hCAR mice to existing transgenic mouse models for prostate cancer (e.g., TRAMP) results in improved mouse models for testing adenovirally-delivered therapeutic genes. (c) 2005 Wiley-Liss, Inc.
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