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Reference: J:27413 Bulfone A, Neuron 1995 Jul;15(1):63-78
Assay type: RNA in situ
MGI Accession ID: MGI:4838637
Gene symbol: Tbr1
Gene name: T-box brain gene 1
Modification date: 11/16/2010

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Probe: Tes-56
Probe preparation: Antisense RNA labelled with S35
Visualized with: Autoradiography

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Specimens Used

	3f	4e	4f	4g	4h	5c	5d	not shown	6e	6f	6g	6h	not shown	7a	7b	7c	not shown	7d	not shown	not shown
Genetic Background	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified
Mutant Allele(s)
Age	E 12.5 (a)	E 12.5 (a)	E 12.5 (a)	E 12.5 (a)	E 12.5 (a)	E 16.5 (a)	E 16.5 (a)	E 16.5 (a)	E 18.5 (a)	E 18.5 (a)	E 18.5 (a)	E 18.5 (a)	P 0	P 1	P 7	P 7	P 17	P adult	P adult	P
Sex	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified
Type	section	section	section	section	section	section	section	section	section	section	section	section	section	section	section	section	section	section	section	section
Fixation	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified
Embedding	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection

Notes:
(a) Age of embryo at noon of plug day not specified in reference.

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Results: 3f (embryonic day 12.5)

Structure	Level	Pattern	Note	Image
TS20: cerebral cortex mantle layer	Present	Regionally restricted	(a)	Figure 3f
TS20: cerebral cortex intermediate zone	Present	Not Specified		Figure 3f
TS20: cortical plate	Present	Not Specified		Figure 3f
TS20: telencephalon ventricular layer	Absent		(b)	Figure 3f

Notes:
(a) Expression in the mantle zone of the cerebral cortex in postmitotic cells.
(b) No expression in the ventricular zone.

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Results: 4e (embryonic day 12.5)

Structure	Level	Pattern	Note	Image
TS20: telencephalon	Present	Regionally restricted	(a)	Figure 4e
TS20: ganglionic eminence	Present	Regionally restricted	(b)	Figure 4e
TS20: thalamus	Absent		(c)	Figure 4e

Notes:
(a) Expression in primordia of paleocortex, neocortex and archicortex. Expression in primordia of anterior olfactory nucleus, diagonal band nucleus, eminentia thalami, and bed nucleus of stria medullaris.
(b) No expression in the lateral ganglionic eminence.
(c) No expression in dorsal thalamus.

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Results: 4f (embryonic day 12.5)

Structure	Level	Pattern	Note	Image
TS20: telencephalon	Present	Regionally restricted	(a)	Figure 4f
TS20: ganglionic eminence	Present	Regionally restricted	(b)	Figure 4f
TS20: choroid invagination	Absent			Figure 4f

Notes:
(a) Expression in primordia of paleocortex, neocortex and archicortex. Expression in primordia of anterior olfactory nucleus and diagonal band nucleus.
(b) No expression in the lateral ganglionic eminence.

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Results: 4g (embryonic day 12.5)

Structure	Level	Pattern	Note	Image
TS20: telencephalon	Present	Regionally restricted	(a)	Figure 4g
TS20: ganglionic eminence	Present	Regionally restricted	(b)	Figure 4g
TS20: thalamus	Absent		(c)	Figure 4g

Notes:
(a) Expression in primordia of paleocortex, neocortex and archicortex. Expression in the eminentia thalami.
(b) No expression in the lateral ganglionic eminence.
(c) No expression in dorsal thalamus.

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Results: 4h (embryonic day 12.5)

Structure	Level	Pattern	Note	Image
TS20: telencephalon	Present	Regionally restricted	(a)	Figure 4h
TS20: ganglionic eminence	Present	Regionally restricted	(b)	Figure 4h

Notes:
(a) Expression in primordia of paleocortex, neocortex and archicortex. Expression in the bed nucleus of stria medullaris.
(b) Expression in the caudal ganglionic eminence.

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Results: 5c (embryonic day 16.5)

Structure	Level	Pattern	Note	Image
TS24: cerebral cortex	Present	Regionally restricted	(a)	Figure 5c
TS24: cortical plate	Present	Regionally restricted	(b)	Figure 5c
TS24: skin	Present	Not Specified		Figure 5c

Notes:
(a) Expression rostral to a boundary in the superficial zone of the neocortical plate, which shifts caudally in more medial regions of the neocortex.
(b) Expression in the superficial cortical plate is 6-fold greater rostrally than caudally. Expression in deep layers was not significantly different.

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Results: 5d (embryonic day 16.5)

Structure	Level	Pattern	Note	Image
TS24: cerebral cortex	Present	Regionally restricted	(a)	Figure 5d
TS24: skin	Present	Not Specified		Figure 5d

Notes:
(a) Expression rostral to a boundary in the superficial zone of the neocortical plate, which shifts caudally in more medial regions of the neocortex.

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Results: not shown (embryonic day 16.5)

Structure	Level	Pattern	Note	Image
TS24: thalamus	Absent		(a)

Notes:
(a) No expression in dorsal thalamus.

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Results: 6e (embryonic day 18.5)

Structure	Level	Pattern	Note	Image
TS26: cortical plate	Present	Regionally restricted	(a)	Figure 6e
TS26: amygdala	Present	Not Specified	(b)	Figure 6e
TS26: forebrain hippocampus	Present	Regionally restricted	(c)	Figure 6e
TS26: skin	Present	Not Specified		Figure 6e

Notes:
(a) Expression rostral to a boundary in the superficial zone of the neocortical plate.
(b) Expression in parts of the the amygdala.
(c) Discontinuity of expression which approximates boundary of hippocampus with subiculum.

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Results: 6f (embryonic day 18.5)

Structure	Level	Pattern	Note	Image
TS26: cortical plate	Present	Regionally restricted	(a)	Figure 6f
TS26: forebrain hippocampus	Present	Regionally restricted	(b)	Figure 6f

Notes:
(a) Expression rostral to a boundary in the superficial zone of the neocortical plate.
(b) Discontinuity of expression which approximates boundary of hippocampus with subiculum.

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Results: 6g (embryonic day 18.5)

Structure	Level	Pattern	Note	Image
TS26: cortical plate	Present	Regionally restricted	(a)	Figure 6g
TS26: cerebral cortex subplate	Present	Not Specified	(b)	Figure 6g
TS26: amygdala	Present	Regionally restricted	(c)	Figure 6g
TS26: forebrain hippocampus	Present	Regionally restricted	(d)	Figure 6g
TS26: thalamus	Absent		(e)	Figure 6g

Notes:
(a) Expression rostral to a boundary in the superficial zone of the neocortical plate.
(b) Expression in a thin layer of cells between the cortical plate and the intermediate zone which may be the subplate.
(c) Expression in parts of the the amygdala.
(d) Discontinuity of expression which approximates boundary of hippocampus with subiculum. Expression in subiculum decreases abruptly at the hippocampal primordium.
(e) No expression in dorsal thalamus.

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Results: 6h (embryonic day 18.5)

Structure	Level	Pattern	Note	Image
TS26: cortical plate	Present	Regionally restricted	(a)	Figure 6h
TS26: amygdala	Present	Regionally restricted	(b)	Figure 6h

Notes:
(a) Expression rostral to a boundary in the superficial zone of the neocortical plate.
(b) Expression in parts of the the amygdala.

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Results: not shown (postnatal day 0)

Structure	Level	Pattern	Note	Image
TS28: cerebral cortex	Present	Not Specified	(a)

Notes:
(a) Expression rostral to a boundary in the superficial zone of the neocortical plate.

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Results: 7a (postnatal day 1)

Structure	Level	Pattern	Note	Image
TS28: olfactory bulb	Present	Regionally restricted	(a)	Figure 7a
TS28: olfactory bulb mitral cell layer	Present	Not Specified	(a)	Figure 7a
TS28: nucleus of diagonal band	Present	Regionally restricted	(b)	Figure 7a
TS28: deep cerebellar nucleus	Present	Not Specified		Figure 7a

Notes:
(a) Expression in the olfactory bulb, largely in a single layer that appears to correspond to mitral cells.
(b) Expression in the vertical limb of the diagonal band.

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Results: 7b (postnatal day 7)

Structure	Level	Pattern	Note	Image
TS28: anterior olfactory nucleus	Present	Not Specified		Figure 7b
TS28: telencephalon	Present	Regionally restricted	(a)	Figure 7b
TS28: neocortex	Present	Not Specified		Figure 7b
TS28: cerebral cortex	Present	Regionally restricted	(b)	Figure 7b
TS28: cerebral cortex layer VI	Present	Not Specified		Figure 7b
TS28: cerebral cortex layer V	Present	Regionally restricted	(c)	Figure 7b
TS28: hippocampus	Present	Regionally restricted	(d)	Figure 7b
TS28: nucleus of diagonal band	Present	Regionally restricted	(e)	Figure 7b
TS28: dorsal thalamus	Present	Not Specified		Figure 7b
TS28: deep cerebellar nucleus	Present	Not Specified		Figure 7b

Notes:
(a) Expression in a thin strip of cells at the interface of white matter and neocortex; may correspond to the subplate.
(b) Much less expression in a zone superficial to layer 6 that may correspond to a sublamina of layer 5.
(c) Expression in scattered cells. Thin line of expressing cells becomes much wider.
(d) Attenuation of expression at the boundary of the subiculum with the hippocampus CA1.
(e) Expression in the horizontal limb of the diagonal band.

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Results: 7c (postnatal day 7)

Structure	Level	Pattern	Note	Image
TS28: neocortex	Present	Regionally restricted		Figure 7c
TS28: cerebral cortex layer V	Present	Regionally restricted	(a)	Figure 7c
TS28: entorhinal cortex	Present	Not Specified		Figure 7c
TS28: anterior olfactory nucleus	Present	Not Specified		Figure 7c
TS28: accessory olfactory bulb	Present	Not Specified		Figure 7c
TS28: nucleus of diagonal band	Present	Regionally restricted	(b)	Figure 7c
TS28: hippocampus	Present	Regionally restricted	(c)	Figure 7c
TS28: dentate gyrus	Strong	Not Specified	(d)	Figure 7c
TS28: hippocampus region CA3	Strong	Not Specified	(d)	Figure 7c
TS28: thalamus	Present	Regionally restricted	(e)	Figure 7c
TS28: dorsal thalamus	Present	Not Specified		Figure 7c

Notes:
(a) Expression in scattered cells. Thin line of expressing cells becomes much wider.
(b) Expression in the vertical limb of the diagonal band.
(c) Attenuation of expression at the boundary of the subiculum with the hippocampus CA1.
(d) High levels of expression in hippocampus beginning at approximately CA3 and continuing into the dentate gyrus.
(e) Expression nucleus of the stria medullaris.

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Results: not shown (postnatal day 17)

Structure	Level	Pattern	Image
TS28: thalamus	Weak	Not Specified

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Results: 7d (postnatal adult)

Structure	Level	Pattern	Note	Image
TS28: endopiriform nucleus	Present	Not Specified		Figure 7d
TS28: piriform cortex	Present	Not Specified		Figure 7d
TS28: telencephalon	Present	Regionally restricted	(a)	Figure 7d
TS28: neocortex	Present	Regionally restricted		Figure 7d
TS28: cerebral cortex layer VI	Present	Not Specified		Figure 7d
TS28: cerebral cortex layer IV	Weak	Not Specified		Figure 7d
TS28: cerebral cortex layer V	Weak	Not Specified		Figure 7d
TS28: cerebral cortex layer I	Strong	Not Specified		Figure 7d
TS28: cerebral cortex layer II	Strong	Not Specified		Figure 7d
TS28: cerebral cortex layer III	Strong	Not Specified		Figure 7d
TS28: claustrum	Present	Not Specified		Figure 7d
TS28: entorhinal cortex	Present	Not Specified		Figure 7d
TS28: hippocampus	Present	Not Specified		Figure 7d
TS28: nucleus of lateral olfactory tract	Present	Not Specified		Figure 7d
TS28: amygdala	Present	Regionally restricted	(b)	Figure 7d
TS28: cerebellum purkinje cell layer	Present	Regionally restricted	(c)	Figure 7d

Notes:
(a) Thin strip of expressing cells between white matter and layer 6 that may correspond to the subplate.
(b) Expression in parts of the amygdala.
(c) Expression in Purkinge cells in the lateral cerebellum.

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Results: not shown (postnatal adult)

Structure	Level	Pattern	Image
TS28: thalamus	Weak	Not Specified

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Results: not shown (postnatal)

Structure	Level	Pattern	Note	Image
TS28: induseum griseum	Present	Not Specified
TS28: subiculum	Present	Not Specified
TS28: taenia tecta	Present	Not Specified
TS28: locus coeruleus	Present	Regionally restricted	(a)

Notes:
(a) Nest of expressing cells in the region of the locus coeruleus.