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Reference: J:93300 Blackshaw S, PLoS Biol 2004 Oct;2(9):E247
Assay type: RNA in situ
MGI Accession ID: MGI:3586043
Gene symbol: Serpini1
Gene name: serine (or cysteine) peptidase inhibitor, clade I, member 1
Modification date: 08/30/2005

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Probe: UI-M-AP0-abm-d-12-0-UI.s1
Probe preparation: Antisense RNA labelled with digoxigenin
Visualized with: Alkaline phosphatase
Assay notes: This data is from Supplemental Table 5, Gene column, row: 41560 serine protease inhibitor 17. After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde prior to processing.

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Specimens Used

	E12	E14	E16	E18	P0/1	P2/3	P4/5	P6/7	P8/10	Adult	CNS	Embryo
Genetic Background	CD-1	CD-1	CD-1	CD-1	CD-1	CD-1	CD-1	CD-1	CD-1	C57BL/6	CD-1	CD-1
Mutant Allele(s)
Age	E 12.0	E 14.0	E 16.0	E 18.0	P 0-1	P 2-3	P 4-5	P 6-7	P 8-10	P adult	P 6	E 16.0
Sex	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Male	Not Specified	Not Specified
Type	section	section	section	section	section	section	section	section	section	section	section	section
Fixation	Fresh Frozen	Fresh Frozen	Fresh Frozen	Fresh Frozen	Fresh Frozen	Fresh Frozen	Fresh Frozen	Fresh Frozen	Fresh Frozen	Fresh Frozen	Fresh Frozen	Fresh Frozen
Embedding	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection

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Results: E12 (embryonic day 12.0; CD-1)

Structure	Level	Pattern	Note	Image
TS20: retina	Weak	Uniform	(a)

Notes:
(a) Expression was panretinal.

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Results: E14 (embryonic day 14.0; CD-1)

Structure	Level	Pattern	Note	Image
TS22: retina	Present	Uniform	(a)	Figure Submitted Image - AH2e14.5100

Notes:
(a) Expression was panretinal.

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Results: E16 (embryonic day 16.0; CD-1)

Structure	Level	Pattern	Note	Image
TS24: retina	Present	Uniform	(a)	Figure Submitted Image - AH2e16.5

Notes:
(a) Expression was panretinal.

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Results: E18 (embryonic day 18.0; CD-1)

Structure	Level	Pattern	Note	Image
TS26: neural retina outer nuclear layer	Present	Not Specified	(a)	Figure Submitted Image - AH2e18.5

Notes:
(a) Expression was detected in the outer neuroblastic layer.

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Results: P0/1 (postnatal day 0-1; CD-1)

Structure	Level	Pattern	Note	Image
TS28: retina outer nuclear layer	Present	Not Specified	(a)	Figure Submitted Image - AH2p0.5, Submitted Image - AH2p1.5

Notes:
(a) Expression was detected in the outer neuroblastic layer.

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Results: P2/3 (postnatal day 2-3; CD-1)

Structure	Level	Pattern	Note	Image
TS28: retina outer nuclear layer	Present	Not Specified	(a)	Figure Submitted Image - AH2p2.5, Submitted Image - AH2p3.5
TS28: retina inner nuclear layer	Present	Regionally restricted	(b)	Figure Submitted Image - AH2p2.5, Submitted Image - AH2p3.5

Notes:
(a) Expression was detected in the outer neuroblastic layer.
(b) Expression was detected in the scleral inner neuroblastic layer.

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Results: P4/5 (postnatal day 4-5; CD-1)

Structure	Level	Pattern	Note	Image
TS28: retina outer nuclear layer	Present	Not Specified	(a)	Figure Submitted Image - AH2p4.5

Notes:
(a) Expression was detected in the outer neuroblastic layer.

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Results: P6/7 (postnatal day 6-7; CD-1)

Structure	Level	Pattern	Image
TS28: retina outer nuclear layer	Present	Not Specified	Figure Submitted Image - AH2p6.5
TS28: retina inner nuclear layer	Present	Not Specified	Figure Submitted Image - AH2p6.5

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Results: P8/10 (postnatal day 8-10; CD-1)

Structure	Level	Pattern	Image
TS28: retina outer nuclear layer	Present	Not Specified	Figure Submitted Image - AH2p8.5
TS28: retina inner nuclear layer	Present	Not Specified	Figure Submitted Image - AH2p8.5

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Results: Adult (postnatal adult; C57BL/6)

Structure	Level	Pattern	Image
TS28: retina outer nuclear layer	Present	Not Specified
TS28: retina inner nuclear layer	Present	Not Specified

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Results: CNS (postnatal day 6; CD-1)

Structure	Level	Pattern	Note	Image
TS28: central nervous system	Present	Not Specified	(a)

Notes:
(a) Expression was widespread.

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Results: Embryo (embryonic day 16.0; CD-1)

Structure	Level	Pattern	Note	Image
TS24: embryo	Present	Not Specified	(a)

Notes:
(a) Expression was widespread.