---

Reference: J:91257 Gray PA, Science 2004 Dec 24;306(5705):2255-2257
Assay type: RNA in situ
MGI Accession ID: MGI:3508867
Gene symbol: Lhx2
Gene name: LIM homeobox protein 2
Modification date: 04/06/2005

---

Probe: MTF#364
Probe preparation: Antisense RNA labelled with digoxigenin
Visualized with: Alkaline phosphatase

---

Specimens Used

	1	2	S7 J Lhx2 (a)
Genetic Background	C57BL	C57BL	C57BL
Mutant Allele(s)
Age	E 10.5	E 10.5 (b)	E 10.5
Sex	Not Specified	Not Specified	Not Specified
Type	whole mount	whole mount	whole mount
Fixation	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde
Embedding	Not Applicable	Not Applicable	Not Applicable

Notes:
(a) Authors did not specify which probe was used when staining this specimen; it is possible MTF#277 (MGI:3506131) was used.
(b) Theiler stage of this specimen assigned by curators from EMAGE.

---

Results: 1 (embryonic day 10.5; C57BL)

Structure	Level	Pattern	Note	Image
TS17: telencephalon	Present	Regionally restricted	(a)	Figure 0364a
TS17: diencephalon	Present	Regionally restricted	(b)	Figure 0364a
TS17: midbrain	Present	Regionally restricted	(c)	Figure 0364a
TS17: hindbrain	Present	Regionally restricted	(d)	Figure 0364a
TS17: future spinal cord	Present	Regionally restricted	(e)	Figure 0364a
TS17: optic cup	Present	Not Specified	(f)	Figure 0364a

Notes:
(a) Expression was detected in dorsal telencephalon.
(b) Expression was detected in dorsal diencephalon.
(c) Expression was detected in dorsal mesencephalon.
(d) Expression was detected in dorsal rhombencephalon.
(e) Expression was detected in dorsal spinal cord.
(f) The tissue was optic vesicle.

---

Results: 2 (embryonic day 10.5; C57BL)

Structure	Level	Pattern	Note	Image
TS18: telencephalon	Present	Regionally restricted	(a)	Figure 0364b
TS18: diencephalon	Present	Regionally restricted	(b)	Figure 0364b
TS18: midbrain	Present	Regionally restricted	(c)	Figure 0364b
TS18: hindbrain	Present	Regionally restricted	(d)	Figure 0364b
TS18: future spinal cord	Present	Regionally restricted	(e)	Figure 0364b
TS18: eye	Present	Regionally restricted	(f)	Figure 0364b

Notes:
(a) Expression was detected in dorsal telencephalon.
(b) Expression was detected in dorsal diencephalon.
(c) Expression was detected in dorsal mesencephalon.
(d) Expression was detected in dorsal rhombencephalon.
(e) Expression was detected in dorsal spinal cord.
(f) The tissue was optic vesicle.

---

Results: S7 J Lhx2 (embryonic day 10.5; C57BL)

Structure	Level	Pattern	Image
TS17: central nervous system	Present	Not Specified	Figure S7 J Lhx2