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Reference: J:91257 Gray PA, Science 2004 Dec 24;306(5705):2255-2257
Assay type: RNA in situ
MGI Accession ID: MGI:3507730
Gene symbol: Tlx3
Gene name: T cell leukemia, homeobox 3
Modification date: 12/03/2004

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Probe: MTF#460
Probe preparation: Antisense RNA labelled with digoxigenin
Visualized with: Alkaline phosphatase

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Specimens Used

	1	2
Genetic Background	C57BL	C57BL
Mutant Allele(s)
Age	E 13.5	P newborn
Sex	Not Specified	Not Specified
Type	section	section
Fixation	4% Paraformaldehyde	4% Paraformaldehyde
Embedding	Cryosection	Cryosection

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Results: 1 (embryonic day 13.5; C57BL)

Structure	Level	Pattern	Note	Image
TS21: head	Present	Not Specified	(a)	Figure MTF# E 460 Image
TS21: central nervous system	Present	Single cells	(b)	Figure MTF# E 460 Image
TS21: cerebral cortex	Absent			Figure MTF# E 460 Image
TS21: corpus striatum	Absent			Figure MTF# E 460 Image
TS21: thalamus	Present	Single cells	(b)	Figure MTF# E 460 Image
TS21: hypothalamus	Absent			Figure MTF# E 460 Image
TS21: midbrain	Present	Single cells	(b)	Figure MTF# E 460 Image
TS21: hindbrain	Present	Single cells	(b)	Figure MTF# E 460 Image
TS21: spinal cord	Present	Single cells	(b)	Figure MTF# E 460 Image
TS21: cranial ganglion	Present	Single cells	(b)	Figure MTF# E 460 Image
TS21: dorsal root ganglion	Present	Single cells	(b)	Figure MTF# E 460 Image
TS21: brain ventricular layer	Absent			Figure MTF# E 460 Image
TS21: retina	Absent			Figure MTF# E 460 Image
TS21: ear	Absent		(c)	Figure MTF# E 460 Image
TS21: olfactory lobe	Absent		(d)	Figure MTF# E 460 Image

Notes:
(a) Expression was detected in neural and non-neural tissue.
(b) Expression was detected in some cells.
(c) The tissue was inner ear and ear structures.
(d) The tissue was olfactory epithelium and/or bulb.

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Results: 2 (postnatal newborn; C57BL)

Structure	Level	Pattern	Note	Image
TS28: head	Present	Not Specified	(a)	Figure MTF# P 460 Image
TS28: central nervous system	Present	Single cells	(b)	Figure MTF# P 460 Image
TS28: cerebral cortex	Present	Single cells	(b)	Figure MTF# P 460 Image
TS28: corpus striatum	Present	Single cells	(b)	Figure MTF# P 460 Image
TS28: thalamus	Present	Single cells	(b)	Figure MTF# P 460 Image
TS28: hypothalamus	Present	Single cells	(b)	Figure MTF# P 460 Image
TS28: midbrain	Present	Single cells	(b)	Figure MTF# P 460 Image
TS28: cerebellum	Present	Single cells	(b)	Figure MTF# P 460 Image
TS28: hindbrain	Present	Single cells	(b)	Figure MTF# P 460 Image
TS28: spinal cord	Present	Single cells	(b)	Figure MTF# P 460 Image
TS28: cranial ganglion	Present	Single cells	(b)	Figure MTF# P 460 Image
TS28: dorsal root ganglion	Present	Single cells	(b)	Figure MTF# P 460 Image
TS28: brain ventricular zone	Absent			Figure MTF# P 460 Image
TS28: retina	Present	Single cells	(b)	Figure MTF# P 460 Image
TS28: ear	Present	Single cells	(c)	Figure MTF# P 460 Image
TS28: olfactory bulb	Absent		(d)	Figure MTF# P 460 Image
TS28: tooth	Present	Single cells	(b)	Figure MTF# P 460 Image

Notes:
(a) Expression was detected in neural and non-neural tissue.
(b) Expression was detected in some cells.
(c) The tissue was inner ear and ear structures. Expression was detected in some cells.
(d) The tissue was olfactory epithelium and/or bulb.