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Reference: J:85473 Corbin JG, Development 2003 Oct;130(20):4895-906
Assay type: Immunohistochemistry
MGI Accession ID: MGI:2676237
Gene symbol: Shh
Gene name: sonic hedgehog
Modification date: 07/18/2008

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Antibody: 5E1
Detection system: Secondary antibody

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Specimens Used

	1B,1C	S1 A	S1 B	S1 C	S1 D
Genetic Background	Not Specified	Not Specified	involves: 129S4/SvJae	Not Specified	Not Specified
Mutant Allele(s)			Nkx2-1tm1Shk/Nkx2-1tm1Shk	Pax6Sey/Pax6+	Pax6Sey/Pax6Sey
Age	E 9.5 (a)	E 9.5	E 9.5	E 9.5	E 9.5
Sex	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified
Type	section	section	section	section	section
Fixation	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde
Embedding	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection

Notes:
(a) 23 somites

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Results: 1B,1C (embryonic day 9.5)

Structure	Level	Pattern	Note	Image
TS15: telencephalon	Present	Regionally restricted	(a)	Figure 1B, 1C

Notes:
(a) Expression in ventral-most telencephalon.

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Results: S1 A (embryonic day 9.5)

Structure	Level	Pattern	Image
TS15: telencephalon	Present	Regionally restricted	Figure S1 A

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Results: S1 B (embryonic day 9.5; involves: 129S4/SvJae; Nkx2-1^tm1Shk/Nkx2-1^tm1Shk)

Structure	Level	Pattern	Image
TS15: telencephalon	Present	Regionally restricted	Figure S1 B

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Results: S1 C (embryonic day 9.5; Pax6^Sey/Pax6⁺)

Structure	Level	Pattern	Image
TS15: telencephalon	Present	Regionally restricted	Figure S1 C

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Results: S1 D (embryonic day 9.5; Pax6^Sey/Pax6^Sey)

Structure	Level	Pattern	Image
TS15: telencephalon	Present	Regionally restricted	Figure S1 D