MGI 5.13 - Gene Expression Data Query Results (Details) (Summary)

Gene Expression Data
Query Results -- Details

Reference: J:85473 Corbin JG, Development 2003 Oct;130(20):4895-906
Assay type: Immunohistochemistry
MGI Accession ID: MGI:2676233
Gene symbol: Nkx2-1
Gene name: NK2 homeobox 1
Modification date: 07/18/2008

Antibody: rabbit anti-Nkx2.1
Detection system: Secondary antibody
Assay notes: Not specified in reference whether the antibody used was mouse anti-Nkx2.1 (MGI:2676171) or rabbit anti-Nkx2.1 (MGI:2676172).

Specimens Used

	1A,1C	1D,1F	1G,1I	2A	2B	2C	2D	2E	2F	3A	3B	3G	3H	S1 A	S1 B
Genetic Background	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	involves: 129S2/SvPas	Not Specified	Not Specified	involves: 129S4/SvJae
Mutant Allele(s)										Pax6^Sey/Pax6⁺	Pax6^Sey/Pax6^Sey	Gsx2^tm1Ssp/Gsx2⁺	Pax6^Sey/Pax6^Sey		Nkx2-1^tm1Shk/Nkx2-1^tm1Shk
Age	E 9.5 (a)	E 10.0 (b)	E 10.5	E 10.5 (c)	E 10.5 (c)	E 10.5 (c)	E 10.5 (c)	E 10.5 (c)	E 10.5 (c)	E 9.5 (d)	E 9.5 (e)	E 10.0 (f)	E 10.0 (f)	E 9.5	E 9.5
Sex	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified
Type	section	section	section	section	section	section	section	section	section	section	section	section	section	section	section
Fixation	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde
Embedding	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection

Notes:
(a) 23 somites
(b) 27 somites
(c) Age was assigned based on somite numbers reported in the reference. 36-38 somites. Theiler stage of this specimen assigned by curators from EMAGE.
(d) Age was assigned based on somite numbers reported in the reference. 22 somites. Theiler stage of this specimen assigned by curators from EMAGE.
(e) Age was assigned based on somite numbers reported in the reference. 22 somites
(f) Age was assigned based on somite numbers reported in the reference. 31-32 somites

Results: 1A,1C (embryonic day 9.5)

Structure	Level	Pattern	Note	Image
TS15: telencephalon	Present	Regionally restricted	(a)	Figure 1A, 1C

Notes:
(a) Expression of Pax6 and Nkx2.1 forms a boundary in the ventral telencephalon.

Results: 1D,1F (embryonic day 10.0)

Structure	Level	Pattern	Note	Image
TS16: telencephalon	Present	Regionally restricted	(a)	Figure 1D, 1F

Notes:
(a) Slight gap between Pax6 and Nkx2.1 expression.

Results: 1G,1I (embryonic day 10.5)

Structure	Level	Pattern	Note	Image
TS17: telencephalon	Present	Regionally restricted	(a)	Figure 1G, 1I

Notes:
(a) Gap in expression of Pax6 and Nkx2.1 expands.

Results: 2A (embryonic day 10.5)

Structure	Level	Pattern	Note	Image
TS18: telencephalon	Present	Regionally restricted	(a)	Figure 2A

Notes:
(a) Not expressed in anterior telencephalon.

Results: 2B (embryonic day 10.5)

Structure	Level	Pattern	Note	Image
TS18: telencephalon	Present	Regionally restricted	(a)	Figure 2B

Notes:
(a) The most anterior expression is at the level of the septum.

Results: 2C (embryonic day 10.5)

Structure	Level	Pattern	Image
TS18: telencephalon	Present	Regionally restricted	Figure 2C

Results: 2D (embryonic day 10.5)

Structure	Level	Pattern	Image
TS18: telencephalon	Present	Regionally restricted	Figure 2D

Results: 2E (embryonic day 10.5)

Structure	Level	Pattern	Image
TS18: telencephalon	Present	Regionally restricted	Figure 2E

Results: 2F (embryonic day 10.5)

Structure	Level	Pattern	Note	Image
TS18: telencephalon	Present	Regionally restricted	(a)	Figure 2F
TS18: diencephalon	Present	Not Specified		Figure 2F

Notes:
(a) No expression at most posterior level of the telencephalon, expression elsewhere.

Results: 3A (embryonic day 9.5; Pax6^Sey/Pax6⁺)

Structure	Level	Pattern	Note	Image
TS16: telencephalon	Present	Regionally restricted	(a)	Figure 3A

Notes:
(a) Expression of Pax6 and Nkx2.1 form a distinct border.

Results: 3B (embryonic day 9.5; Pax6^Sey/Pax6^Sey)

Structure	Level	Pattern	Note	Image
TS15: telencephalon	Present	Regionally restricted	(a)	Figure 3B

Notes:
(a) Expression does not expand dorsally.

Results: 3G (embryonic day 10.0; involves: 129S2/SvPas; Gsx2^tm1Ssp/Gsx2⁺)

Structure	Level	Pattern	Image
TS16: telencephalon	Present	Regionally restricted	Figure 3G

Results: 3H (embryonic day 10.0; Pax6^Sey/Pax6^Sey)

Structure	Level	Pattern	Image
TS16: telencephalon	Present	Regionally restricted	Figure 3H

Results: S1 A (embryonic day 9.5)

Structure	Level	Pattern	Image
TS15: telencephalon	Present	Regionally restricted	Figure S1 A

Results: S1 B (embryonic day 9.5; involves: 129S4/SvJae; Nkx2-1^tm1Shk/Nkx2-1^tm1Shk)

Structure	Level	Pattern	Image
TS15: telencephalon	Present	Regionally restricted	Figure S1 B

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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