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Reference: J:85473 Corbin JG, Development 2003 Oct;130(20):4895-906
Assay type: Immunohistochemistry
MGI Accession ID: MGI:2676225
Gene symbol: Pax6
Gene name: paired box gene 6
Modification date: 10/08/2003

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Antibody: PRB-278P
Detection system: Secondary antibody
Assay notes: Not specified in reference whether the antibody used was mouse anti-Pax6 (MGI:2447626) or rabbit anti-Pax6 (MGI:2157328).

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Specimens Used

	1A,1C	1D,1F	1G,1H,1I	3C	3D	6E,6M	6F,6N	6G,6O	6H,6P
Genetic Background	Not Specified	Not Specified	Not Specified	Not Specified	involves: 129S4/SvJae	Not Specified	involves: 129S2/SvPas	involves: 129S4/SvJae	involves: 129S2/SvPas * 129S4/SvJae
Mutant Allele(s)					Nkx2-1tm1Shk/Nkx2-1tm1Shk		Gsx2tm1Ssp/Gsx2tm1Ssp	Nkx2-1tm1Shk/Nkx2-1tm1Shk	Gsx2tm1Ssp/Gsx2tm1Ssp Nkx2-1tm1Shk/Nkx2-1tm1Shk
Age	E 9.5 (a)	E 10.0 (b)	E 10.5	E 9.5 (c)	E 9.5 (c)	E 12.5	E 12.5	E 12.5	E 12.5
Sex	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified
Type	section	section	section	section	section	section	section	section	section
Fixation	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde	4% Paraformaldehyde
Embedding	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection	Cryosection

Notes:
(a) 23 somites
(b) 27 somites
(c) Age was assigned based on somite number reported in reference. 22 somites

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Results: 1A,1C (embryonic day 9.5)

Structure	Level	Pattern	Note	Image
TS15: telencephalon	Present	Regionally restricted	(a)	Figure 1A, 1C

Notes:
(a) Expression of Pax6 and Nkx2.1 forms a boundary in the ventral telencephalon.

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Results: 1D,1F (embryonic day 10.0)

Structure	Level	Pattern	Note	Image
TS16: telencephalon	Present	Regionally restricted	(a)	Figure 1D, 1F

Notes:
(a) Slight gap between Pax6 and Nkx2.1 expression.

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Results: 1G,1H,1I (embryonic day 10.5)

Structure	Level	Pattern	Note	Image
TS17: telencephalon	Present	Regionally restricted	(a)	Figure 1G, 1H, 1I

Notes:
(a) Gap in expression of Pax6 and Nkx2.1 expands.

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Results: 3C (embryonic day 9.5)

Structure	Level	Pattern	Note	Image
TS15: telencephalon	Present	Regionally restricted	(a)	Figure 3C

Notes:
(a) Expression of Pax6 and Nkx2.1 form a distinct border.

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Results: 3D (embryonic day 9.5; involves: 129S4/SvJae; Nkx2-1^tm1Shk/Nkx2-1^tm1Shk)

Structure	Level	Pattern	Note	Image
TS15: telencephalon	Present	Regionally restricted	(a)	Figure 3D

Notes:
(a) Expression does not expand ventrally.

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Results: 6E,6M (embryonic day 12.5)

Structure	Level	Pattern	Note	Image
TS20: telencephalon	Present	Regionally restricted	(a)	Figure 6E, 6M

Notes:
(a) Expression normally extends just across the cortical sulcus into the dorsal lateral ganglionic eminence.

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Results: 6F,6N (embryonic day 12.5; involves: 129S2/SvPas; Gsx2^tm1Ssp/Gsx2^tm1Ssp)

Structure	Level	Pattern	Note	Image
TS20: telencephalon	Present	Regionally restricted	(a)	Figure 6F, 6N

Notes:
(a) Expression expands ventrally into the lateral ganglionic eminence.

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Results: 6G,6O (embryonic day 12.5; involves: 129S4/SvJae; Nkx2-1^tm1Shk/Nkx2-1^tm1Shk)

Structure	Level	Pattern	Note	Image
TS20: telencephalon	Present	Regionally restricted	(a)	Figure 6G, 6O

Notes:
(a) Expression does not expand into the subpallium.

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Results: 6H,6P (embryonic day 12.5; involves: 129S2/SvPas * 129S4/SvJae; Gsx2^tm1Ssp/Gsx2^tm1Ssp, Nkx2-1^tm1Shk/Nkx2-1^tm1Shk)

Structure	Level	Pattern	Note	Image
TS20: telencephalon	Present	Regionally restricted	(a)	Figure 6H, 6P

Notes:
(a) Significant expansion into ventral-most telencephalon.