References
Query Results -- Details
MGI Accession ID: MGI:49242
J Number: J:708
Other Accession IDs:
Title: Characterization of mouse-hamster somatic cell hybrids by PCR: a panel of mouse-specific primers for each chromosome.
Authors: Abbott C
Journal: Mamm Genome
Volume: 2
Issue: 2
Date: 1992
Year: 1992
Pages: 106-9
Review Status: Peer Reviewed
Abstract:
Mouse/hamster somatic cell hybrids form a valuable resource for mouse gene mapping. Characterization of these hybrids by isozyme analysis can be technically demanding and time-consuming. Species-specific polymerase chain reaction (PCR), where a mouse gene but not its homolog in the hamster is amplified, can provide an alternative means of characterization. Mouse-specific primers have been designed for at least one gene on each of the mouse autosomes and the X Chromosome (Chr). Primers are chosen to correspond to untranslated regions of the mouse gene concerned, in order to decrease the chance of cross-hybridization with the homologous hamster gene. These primer sequences are presented, together with the conditions for their use.
