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| Nomenclature |
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Symbol:
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Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze
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Name:
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gene trap ROSA 26, Philippe Soriano;
targeted mutation 6, Hongkui Zeng
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MGI ID: |
MGI:3809522 |
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Synonyms: |
Ai6, Rosa-CAG-LSL-ZsGreen1-WPRE |
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Gene:
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Gt(ROSA)26Sor
Location:
unknown
Genetic Position: Chr6,
48.7 cM
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Mutation
origin |
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Germline Transmission:
| Earliest citation of germline transmission:
J:23000
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Parent Cell Line:
| G4 (ES Cell) |
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Strain of Origin:
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(129S6/SvEvTac x C57BL/6NCr)F1
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Mutation
description |
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Allele
Type: |
Targeted (knock-in) |
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Mutation: |
Insertion |
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The Rosa-CAG-LSL-ZsGreen1-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), ZsGreen1 sequence (from pZsGreen1-N1 (Clontech); a human codon optimized/enhanced green fluorescent protein engineered for brighter fluorescence and higher expression in mammalian cells), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. |
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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